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28 Sep 2019
We did the following experiments in the lab ..construct your LepA/EF-4 GFP fusion. Write the introduction part about those experiments, explaint/introduce what are they? the purpose? background of each experiment? outline your hypothesis!
o pPEM109 plasmid purification (target DNA)
o GFP PCR (donor DNA) and column clean-up
o Transposition reaction
o Transformation of plasmid library into E. coli
o Selecting GFP positive colonies and re-streaking for single colonies o Inoculation of GFP positive colony into LB+AMP liquid cultures minus IPTG
o Plasmid purification and sequencing
o Mapping of GFP structure onto PDB molecule model of LepA/EF-4
We did the following experiments in the lab ..construct your LepA/EF-4 GFP fusion. Write the introduction part about those experiments, explaint/introduce what are they? the purpose? background of each experiment? outline your hypothesis!
o pPEM109 plasmid purification (target DNA)
o GFP PCR (donor DNA) and column clean-up
o Transposition reaction
o Transformation of plasmid library into E. coli
o Selecting GFP positive colonies and re-streaking for single colonies o Inoculation of GFP positive colony into LB+AMP liquid cultures minus IPTG
o Plasmid purification and sequencing
o Mapping of GFP structure onto PDB molecule model of LepA/EF-4
Deanna HettingerLv2
28 Sep 2019
