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1. How would you expect the zone of inhibition to change in a Kirby Bauer test if the antibiotic concentration added to the filter paper was doubled?

A. Larger B. Smaller C. Stay the same

2. How would you expect the zone of inhibition to change in a Kirby Bauer test if the agar depth was doubled?

A. Larger B. Smaller C. Stay the same

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Patrina Schowalter
Patrina SchowalterLv2
28 Sep 2019

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Related questions

I need help with the discussion and conclusion. This is the correct result from the experiment and correct format for the lab report.

Objective

Antibiotic disc test (Perform antibiotics test assay)

Introduction

The purpose of this experiment is to learn how to test the susceptibility of microorganisms to various antibiotics. Antibiotic is a chemical produced by a fungus or bacterium that has the potential to limit the growth of another bacterium.

Material and Methods

The description is in the laboratory manual. Test 1 using the aseptic technique to paint the entire surface of a sterile agar plate with E. coli and to covers all the areas then place four different antibiotics disc onto the marked surface with their proper name onto it.

Results

Table 1: Antibiotic inhibition zone results for E. coli

Diameter – Disc/2 = Radius (r)

Disc = 6mm/10 = 0.6 cm.

Bacterium: E. coli

Antibiotic used

Inhibition zone size (cm)

Penicillin 10 mg/1

0

Ampicillin

3.8 – 0.6/2 = 1.6

Penicillin 100 mg/1

1.70 – 0.6/2 = 0.55

Cefoxitin

4.20 – 0.6/2 = 1.8

Discussion/conclusion

. A child with very low blood oxygen levels was given a test foralpha thalassemia The test was a Northern blot test using astandard probe that binds to alpha globin mRNA. The resultingNorthern blot had three bands, indicating the child has threedifferent sizes of alpha globin mRNA molecules. One is normal size,one is larger than normal, and one is smaller than normal. Theparents of the patient are both from Mediterranean countries butthere is no reasonable chance that they are related.

a). Give one possible mutant genotype that the child might havethat would result in her forming three different sized alpha globinmRNA molecules, and explain how this genotype would also cause herto have low blood oxygen levels.

b). What test would you do to confirm your hypothesis?

c). Predict what results you would expect from your test if yourhypothesis is correct

Use the following to answer questions 48-51:

A second amniocentesis is performed on a diabetic woman believed to be at approximately 35 weeks gestation. She is pregnant with her second child, and the bilirubin A450 on the first amniocentesis was in zone 2 of the Liley graph.

48. Why was a second amniocentesis performed?

A) Amniocentesis was in zone 2

B) To determine fetal antibodies

C) To monitor the bilirubin concentration

D) Both A and C

49. If the bilirubin A450 is now in zone 3, what additional testing should be performed?

A) Alpha fetoprotein

B) Fluorescence polarization assay

C) Maternal antibody screen

D) Fern test

50. Should an L/S ratio or an Amniostat test be performed?

A) L/S ratio

B) Amniostat

51. The presence of what substance is of particular concern prior to inducing labor?

A) Lecithin

B) Sphingomyelin

C) Acetylcholinesterase

D) Phosphatidyl glycerol

52. A fern test is performed to differentiate between:

A) phosphatidyl glycerol and lecithin

B) urine and amniotic fluid

C) sphingomyelin and phosphatidyl glycerol

D) lamellar bodies and meconium

53. Failure of the fetus to begin swallowing will result in:

A) oligohydramnios

B) a positive fern test

C) polyhydramnios

D) umbilicord compression

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