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Method involved in fingerprinting ? The DNA sequences generally used for fingerprinting? The Probes used for this protocol? RE’s, RFLP’s, PCR? how to Solving of a crime scene and paternity dispute case?

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Casey Durgan
Casey DurganLv2
28 Sep 2019

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Related questions

DNA Fingerprinting III: Southern Blotting Questions

A) Why do different individuals such as siblings have differentrestriction enzyme recognition sites?

B) What is the function of probes in DNA praternityanalysis?

C) Why is there more than one single locus probe used in anactual paternity DNA test?

D)What advantage does Southern Blot Analysis affer for genetictesting?

Thank you very much!

Protocol-Southern blotting technique ?Probes, RE’s, PCR –need to understand how these are required for this experiment? How depurination and denaturation are carried out for this experiment and why it is necessary for this experiment ? What is the mutation causing sickle cell anemia, what are the other mutation causing structural variation in the hemoglobin protein?

Read the “Today’s Biology” article titled “DNA and the Innocence Project on pg. 267. Research and find another example of DNA fingerprinting reversing a court ruling in which an innocent person was imprisoned for a crime. Summarize that situation and provide a link or two to the case and discuss ways to prevent these cases from occurring.

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