What is the purpose of cell viability testing and what are the steps to perform a cell viability test? This was done using GBM cells and drugs and Vita orange.

answer

watching

views

For unlimited access to Homework Help, a Homework+ subscription is required.

You have 0 free answers left.
Get unlimited access to 3.8 million step-by-step answers.

Get unlimited access

Already have an account? Log in

Elin HesselLv2

30 Sep 2019

Unlock all answers

Get 1 free homework help answer.

Already have an account? Log in

Related questions

A sample of cell suspension is ready for counting and viability assessment; however, after loading the hemocytometer and microscopically observing the cells on the counting grids, it is determined that the counting ranges are too high (i.e. there are too many cells to count). What must be done to obtain good counting ranges? What volume of medium must be added to 0.1 ml of the original cell suspension to obtain a 1:10 dilution?

ochregoose404

Two T-75 flasks containing anchorage-dependent cultures were trypsinized. The cells from both flasks were pooled, and the resulting cell suspension was transferred to a conical tube. The total volume of liquid cells suspended in media was 20.5 mls. Although these cells are routinely split 1:2, anyone handling these cultures is requested to assess the viability of the population. 0.5 ml of the cell suspension was added to a tube containing an equal volume of trypan blue, the tube was agitated and hemocytometer was loaded with the resulting suspension. Both grids were covered with appropriate amount of cell suspension, and the following counts were obtained. Grid #1: 5 squares counted for a total of 176 cells (5 out of 176 cells were stained blue) Grid #2: 5 squares counted for a total of 195 cells. (5 out of 195 cells were stained blue) What is the average number of viable cell per square? What is the % viability? What is the total number of cells available for subculturing? Since these cells are routinely split 1:2, approximately how many millions of cells will be used to seed each of the 4 flasks?

ceruleanclam711

3. A sample of cell suspension is ready for counting and viability assessment; however, after loading the hemocytometer and microscopically observing the cells on the counting grids, it is determined that the counting ranges are too high (i.e. there are too many cells to count). What must be done to obtain good counting ranges? What volume of medium must be added to 0.1 ml of the original cell suspension to obtain a 1:10 dilution? What is the dilution factor? If a sample of this 1:10 dilution is added to an equal volume of trypan blue solution, what is the final dilution factor?

roselouse287

Weekly leaderboard

Home

Homework Help 3,900,000

Biology 460,000

What is the purpose of cell viability testing and what are the steps to perform a cell viability test? This was done using GBM cells and drugs and Vita orange.

Unlock all answers

Related textbook solutions

Molecular Cell Biology

Biology: Science for Life with Physiology

Concepts of Biology

Biology

Essentials of Biology

Related questions

Weekly leaderboard