Prelab 9: Selective Media Read pages 227-230 and 241-256 in yourlab manual, watch the video, and answer the following questions inyour own words before coming to lab. Chapter 6 in your textbook mayalso be helpful. You may also want to review the video oninoculation techniques. (I will hand out a written procedure forthe KOH test in lab.) NOTE: for all these procedures over the nextfew weeks, you will be testing only your assigned organism on thegiven media. 1. Why are plates inverted (agar side up) whilethey're incubated? 2. When picking up bacteria growing on a solidmedium to transfer to a new culture, do you need to scrape up ahuge visible amount? Explain. 3. Instead of incubating a separatecontrol plate, you will leave a space on your plate uninoculated asa control. One reason for this control is to show what color themedium is without microbial growth; why is this necessary? What isa second thing that this control tells you about theexperiment?
Prelab 9: Selective Media Read pages 227-230 and 241-256 in yourlab manual, watch the video, and answer the following questions inyour own words before coming to lab. Chapter 6 in your textbook mayalso be helpful. You may also want to review the video oninoculation techniques. (I will hand out a written procedure forthe KOH test in lab.) NOTE: for all these procedures over the nextfew weeks, you will be testing only your assigned organism on thegiven media. 1. Why are plates inverted (agar side up) whilethey're incubated? 2. When picking up bacteria growing on a solidmedium to transfer to a new culture, do you need to scrape up ahuge visible amount? Explain. 3. Instead of incubating a separatecontrol plate, you will leave a space on your plate uninoculated asa control. One reason for this control is to show what color themedium is without microbial growth; why is this necessary? What isa second thing that this control tells you about theexperiment?
