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Amino acids that make up the active site of enzymes can be farapart in the primary sequence because ____.

the primary sequence can be rearranged once the protein issynthesized
folding the protein can bring them close together
the substrate can be passed along the primary sequence to theappropriate positions
the amino acids cannot be far apart on the primary sequence,they must be close

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Hubert Koch
Hubert KochLv2
30 Sep 2019

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3.Prior to export from the nucleus, pre-mRNA must be processed. What specifically does this entail?

4.The biosynthetic pathway for the two amino acids E and H is shown schematically in the figure. You are able to show experimentally that E inhibits enzyme V, and H inhibits enzyme X. The most logical hypothesis regarding the regulation of Enzyme T would be that it is most likely subject to feedback inhibition by E alone. True or False?

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6. Looking at the figure showing the change in reaction rate for two different enzymes that both utilize the same substrate, answer the following questions. a. What is the approximate Vmax for enzyme B ? b. What is the approximate Vmax for enzyme A ? c. What is the approximate Km for the enzyme which has a higher affinity for the substrate uM.

7. Answer the following question either yes or no (spell out the word, do not abbreviate as y or n). Looking at the following coupled reaction: Luciferase luciferin + O 2 ==========> oxyluciferin + light ∆G = + + Mg2+ ATP <=> AMP + PPi ∆G = - - - a. Based on your knowledge of free energy change and reactions would this coupled reaction occur ? b. Would changing the second step from ATP<=> AMP + PPi ∆G = - - - to ATP <=> ADP + Pi ∆G = - change your answer

7.Spontaneous reactions by definition will happen instantaneously. Why false?

8. Each of the following questions apply to the image of the channel protein. The purple sphere is a charged ion. Type your response to each question in the blank provided. a. Based you on what you know about cells and ions, what does the tri-lobed red and white molecule represent (although there aren't anywhere near enough of them shown) ? b. Since the question stated the purple sphere was a charged particle, using what you know about the tri-lobed red and white molecule what is the charge of the purples sphere c. On the extracellular space, on the right side of the purple sphere, there are two tri-lobed red and white molecules in close proximity to one another. The red of one appears to be attracted to the white of the other. What type of bond is this ? d. Based on your knowledge of proteins that interact with the membrane and the properties of the membrane, to what family of amino acids would the amino acid marked by A. most likely belong? ?

2. Explain the structural role of the four different categories of biomolecules within a cell.

3. Explain how water, a steroid hormone, and a monosaccharide are transported into a cell down their concentration gradient and explain why the different molecules are transported in different ways.

4. Explain which forms of transport require energy and why, and how ATP facilitates energy-dependent transport.

5. All of the different types of biomolecules can serve as energy sources. Explain which biomolecules are catabolized in which metabolic pathways.

6. Whether or not the cellular machinery is in place for particular catabolic reactions to go forward depends on which genes are being expressed. Explain the different points, in a eukaryotic cell, at which gene expression might be regulated, and how.

7. The primary tasks of any organism are survival and reproduction. Acquiring biomolecules and catabolizing them to generate energy is a major factor in survival. For reproduction to occur, DNA must first be replicated so that it can be passed on. Describe the process of DNA replication.

8. If you wanted to replicate DNA artificially in large quantities, how would you go about it?

9. Once DNA has been replicated, what is the sequence of events in the cell cycle and in cell division that produce the gametes for sexual reproduction? Where does this event fit, along with other cell cycles, in the life cycle of the organism?

10. A number of physical traits in humans are controlled by a simple dominant/recessive relationship between two alleles; here are some examples (dominant/recessive):

Widow’s peak/no widow’s peak

Free earlobes/attached earlobes

Cleft chin/no cleft chin

Dimples/no dimples

Freckles/no freckles

Naturally curly hair (really curly, not wavy)/straight hair

Make a pedigree of your immediate family indicating the possible genotype of each family member (for the six genes listed above) based on their phenotype. If you are adopted, indicate the possible genotypes and phenotypes of your parents. You may make this part up.

Question 1 When energy changes from one form to another, some of the energy that can be used to do work is always lost. The loss of this energy is mostly in the form of _________.
a. heat
b. potential energy
c. kinetic energy
d. light energy
e. mechanical energy

Question 2 Which of the following is exergonic?
a. binding fatty acids and glycerol molecules together to form lipids
b. building proteins from amino acids
c. binding sugars molecules together to form glycogen
d. breaking bonds in starch to release glucose
e. adding a phosphate group to ADP to form ATP

Question 3 The first law of thermodynamics states that:
a. energy can neither be created nor destroyed.
b. the universe is increasing in entropy.
c. all living organisms must eat to derive energy.
d. energy is required to bring molecules into a cell against a concentration gradient.
e. in energy-yielding reactions, matter goes from a more-ordered to a less-ordered state.

Question 4 Which of the following is true of energy?
a. It can be transferred from one form to another with 100 percent efficiency.
b. It can be created.
c. It can be stored in chemical bonds.
d. It can never be stored.
e. It can be released by forming chemical bonds.

Question 5 Metabolism is:
a. the sum of all the energy a cell uses.
b. the sum of all the vitamins a cell contains.
c. the sum of all the enzymes a cell contains.
d. the sum of all the chemical reactions that an organism carries out.
e. the sum of all the ATP a cell contains.

Question 6 Entropy is the measure of ________ in a system.
a. disorder
b. order
c. energy
d. work
e. heat

Question 7 What percentage of the energy stored in glucose are humans cells able to use to do useful work?
a. 37%
b. 100%
c. 15%
d. 52%
e. 48%

Question 8 What is activation energy?
a. the energy gained by living things, allowing them to remain active
b. the energy produced by the breakdown of ATP to ADP.
c. the energy required to initiate or begin a chemical reaction
d. the energy created by enzymes during exergonic reactions
e. the energy lost by living things in the form of heat

Question 9 Which of the following is true of allosteric enzymes?
a. Their activity can be reduced when the product binds the enzyme.
b. Their activity can be reduced when a coenzyme binds to them.
c. Their activity can be reduced when the substrate binds the enzyme.
d. Their activity can be increased when the product binds the enzyme.

Question 10 Which of the following is a true statement about enzymes?
a. They are made of proteins.
b. They are altered by the reactions they catalyze.
c. They increase the energy of activation of chemical reactions.
d. All of the above are true.
e. None of the above is true.

Question 11 What is the energy currency of cells?
a. ADP
b. enzymes
c. glucose
d. vitamins
e. ATP

Question 12 Kinetic energy is ____________, while potential energy is _____________.
a. stored energy; energy that is being used
b. stored energy; energy that can't be used
c. energy that is being used; stored energy
d. energy that can't be used; stored energy

Question 13 The energy released during ________ reactions can be used to drive ________ reactions, and this is called ________ reactions.
a. exergonic; endergonic; coupled
b. endergonic; exergonic; coupled
c. exergonic; endergonic; mutually dependent
d. coupled; mutually dependent; independent
e. endergonic; exergonic; mutually dependent

Question 14 ADP + P + Energy --> ATP Which of the following is a true statement about the chemical reaction above?
a. This is a coupled reaction.
b. This is an exergonic reaction.
c. The product of the reaction contains more energy than that of all of the reactants combined.
d. This is an endergonic reaction.
e. This is an example of a catabolic reaction.

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