This summer you interned in a lab that works with mice. Younotice that a mutation has arisen in your mouse colony that leadsto significantly increased susceptibility to infections. Most ofthese mice die early in life due to infections. You perform a quickassay of the blood and find there are no antibodies present.
a). (5 points) Describe briefly how you could show whether allleukocytes are absent, only lymphocytes, only T cells, or only Bcells?
b). (10 points) Describe an experiment you could use to identifyif the defect is in these mice is in their bone marrowâshematopoietic cells OR non-hematopoietic cells (e.g. stromal orreticular cells). Be sure to include appropriate controls and adescription of what you would expect to see for each possibility(in other words, what would you expect to see if the defect was inHSCs? What would you expect to see if the defect is in othernon-hematopoietic bone marrow cells?
This summer you interned in a lab that works with mice. Younotice that a mutation has arisen in your mouse colony that leadsto significantly increased susceptibility to infections. Most ofthese mice die early in life due to infections. You perform a quickassay of the blood and find there are no antibodies present.
a). (5 points) Describe briefly how you could show whether allleukocytes are absent, only lymphocytes, only T cells, or only Bcells?
b). (10 points) Describe an experiment you could use to identifyif the defect is in these mice is in their bone marrowâshematopoietic cells OR non-hematopoietic cells (e.g. stromal orreticular cells). Be sure to include appropriate controls and adescription of what you would expect to see for each possibility(in other words, what would you expect to see if the defect was inHSCs? What would you expect to see if the defect is in othernon-hematopoietic bone marrow cells?
