answer

watching

views

25 Mar 2018

Q: Compare and contrast the different types of microscopy?

Q: For each of the following types of light microscopes briefly describe it; state some specific uses; state some limitations of use (if applicable):

(a) Bright field

(b) Dark field

(c) Phase Contrast

(d) Fluorescent

(e) SEM

(f) TEM

Please help. Thanks!

answer

watching

119

views

For unlimited access to Homework Help, a Homework+ subscription is required.

You have 0 free answers left.
Get unlimited access to 3.8 million step-by-step answers.

Get unlimited access

Already have an account? Log in

Hubert KochLv2

27 Mar 2018

Unlock all answers

Get 1 free homework help answer.

Already have an account? Log in

Related questions

26.The best microscopy technique for observing different subcellular structures on the surface of bacteria is

(A) SEM (B) TEM

(E) fluorescence microscopy

27.Which type of microscopy exploits differences in the refractive index of the specimen, where light is retarded by one-quarter of a wavelength when it passes through a bacterial cell?

(A) SEM (B) TEM

(E) fluorescence microscopy

28.Which type of microscopy should be used to examine bacterial cells that have been Gram stained?

(A) TEM (B) fluorescence microscopy

(E) bright-field microscopy

29.Which of the following is most abundant in a prokaryotic cell (in dry weight)?

(A) RNA (B) DNA

(E) Lipid

30.Which of the following techniques uses the Svedberg unit (S)?

(A) electron microscopy (B) ultracentrifugation

(E) sonication

31.Which of the following is NOT a function performed by the cell membrane or its components?

(A) production of proteins (B) detection of environmental signals

(E) B and D

32.Which of the following statement is true?

(A) Lipopolysaccharides (LPS) are found in the cell wall of Gram-negative bacteria.

(B) Lipopolysaccharides (LPS) are found in the cell wall of Gram-positive bacteria.

(D) Teichoic acids are found in the cell wall of Gram-positive bacteria.

(E) Cholesterol is found in the cell wall of Gram-negative bacteria.

33.Which of the following is found in Archaeabut not in Bacteria?

(A) Hopanoids (B) Terpenoids

(E) Peptidoglycan

34.The glycan chain of archaeal cell wall is made of __________ and __________, while the glycan chain of bacterial cell wall is made of __________ and __________.

(A) peptidoglycan and murein, pseudopeptidoglycan and murein

(B) N-acetylglucosamine and N-acetyltalosaminuronic acid,

N-acetylmuramic acid and N-acetyltalosaminuronic acid

N-acetylglucosamine and N-acetylmuramic acid

(D) N-acetylglucosamine and N-acetylmuramic acid,

N-acetylglucosamine and N-acetyltalosaminuronic acid

(E) N-acetylmuramic acid and N-acetyltalosaminuronic acid,

N-acetylglucosamine and N-acetyltalosaminuronic acid

35.The sacculus of bacteria is composed primarily of

(A) phospholipids (B) peptidoglycans

(E) nucleic acids

36.Lysozyme attacks the following linkage in the bacterial cell wall:

(A) Î²(1,4)-glycosidic bonds

(B) Î²(1,3)-glycosidic bonds

(D) Peptide cross-links between glycan chains

(E) Peptide bonds between L-alanine and D-glutamic acid

For each of the following, determine the type of microscopy would be the most useful in the situation described from the following types of microscopy â light, fluorescent, TEM, SEM a. Observe the movement of sperm cells through media. b. Determine whether a membrane localized protein is in the ER or the plasma membrane. c. Examine the shape of a pollen grain. d. Determine the thickness between the plasma membranes of two adjacent cells. e. Compare the ER between two different cell types.

Suppose during your field work, you identified a new single celled organism that you canât immediately determine if itâs eukaryotic or prokaryotic. Which of the following analysis would be the most useful in helping you determine what class of cell you have discovered? Give a 1 sentence explanation. A. Analysis of amino acids present. B. Analysis of types of macromolecules present. C. Analysis of genome size. D. Presence of ribosomes

plumcrow577

Q1. Which of the following is false concerning the Calvin Benson Cycle.

A. Utilizes both NADPH and ATP

B. Requires ribulose bisphophate carboxylase

C. Is responsible for the fixation of carbon dioxide.

D. Oxidize carbons

E. All of the above are true

Q2. Concerning the avian-adapted, highly pathogenic strain of H5N1 (called HPAI A(H5N1), for "highly pathogenic avian influenza virus of type A of subtype H5NI"), which of the following is false:

A. Cause "avian influenza" or simply "bird flu"

B. Has resulted in death of many millions of chickens and birds

C. Has resulted in recent deaths of many hundred thousands of exposed humans.

D. Endemic in many bird populations.

E. All of the above are true

Q3. Which of the following is mismatched concerning microscopy.

A. SEM: 3D representatio of surface features of cells and other objects, individual components in microbial communities, microbial environmental interactions.

B. Confocal: uses laser light sources to scan differentilly lableded specimens producing 3D images assembled by a computer.

C. Darkfield: can visualize living spirochetes in wetmounts, motility of celss in wet mounts.

D. Differential Interference Phase Contrast: Similar to phase contrast but adds prisms providing additional detail and quasi-3D effect.

E. Limit of resolution human eye (conventional light microscopy): 0.2 mm (2nm)

Q4. Which of the following is the correct decreasing order in resistance to heat and chemicals?

A. Protozoan cysts - prions - endospores - fungi - mycobacteria - lipid viruses - E.Coli

B. Endospores - prions - fungi - mycobacteria - protozoan cysts - E.Coli - lipid viruses

C. Endospores - prions - protozoan cysts - fungi - mycobacteria - lipid viruses - E. Coli

D. Prions - endospores - mycobacteria - protozoan cysts - fungi - E.Coli - lipid viruses

E. Prions - endospores - mycobacteria - fungi - protozoan cysts - lipid viruses - E.Coli

Q5. What is false concerning capnophiles?

A. mammalian cells in culture

B. Neisseria

C. Have a requirement for increased carbon dioxide

D. Hemophilus

E. All of the above are true

Q6. Which of the following is false concerning Biosafety Levels?

A. Requirments for facilities increase from BSL- 1 to BSL- 4

B. PPE increses from BSL- 1 to BSL- 4

C. BSL- 4 agents have no vaccines and treatments and include Ebola and Marburg viruses

D. BSL- 3 agents include Vibrio cholerae, Corynebacterium diphtheriase & Borrelia burdorferi

E. all of the above are true.

Q7. Which of the following would be the most likely and available choice for sterilizing heat labile liquids in different research and teaching labs at a school, while still retaining the materials in a useable state?

A. Membrane filtration

B. Gamma radiation

C. Autoclaving

D. Hot air oven

E. HEPA filter

Q8. In the aerobic metabolism of proteins by chemoheterotrophs (e.g., E.Coli and you)

A. Proteins are broken down into smaller prptides and eventually into amino acids by proteses (peptidases)

B. Certain amino acids may be converted to pyruvate

C. Certain amino acids may be converted to intermediates (e.g. oxaloacetate) of the Krebs cycle.

D. Certain amino acids may be converted to acetyl-CoA

E. All of the above are true.

Q9. Which of the following would be the most effective for sterilizing air in a hospital surgical suite during an operation?

A. Membrane Filtration

B. Ethylene oxide gas

C. Autoclaving

D. HEPA filtration

E. Gamma irradiation

Q10. The "roadblock" to continued oxidation of glucose in fermentations is bypassed through production of fermentation products for the purpose of

A. oxidation of NAD+ back to NADH

B. reduction of NAD+ back to NADH

C. reduction of NADH back to NAD+

D. oxidation of NADH back to NAD+

E. generating additional ATPs beyond that produced by glycolysis.

Q11. Which of the following is used in hand sanitizing soaps purchased from your local HEB?

A. Formaldehyde

B. Glutaraldehyde

C. Triclosan

D. Mercury

E. 5% hypochlorite

Q12. Enteric plating media (e.g., MacMonkey and EMB agars) have which sugar and a pH indicator to distinguish coliforms from non coliforms?

A. Glucose

B. Sucrose

C. Lactose

D. Maltose

E. Fructose

Q13. Normal antimicrobial therapy for active tuberculosis involves:

A. one drug for 10 days

B. two or more drugs for 10 days

C. one drug for at least 6 months

D. three or four drugs for at least 6 months

E. six or more drugs for at least 3 years.

Can you guys help me answer these questions please!!!

fuchsiaplatypus869

Weekly leaderboard

Home

Homework Help 3,900,000

Biology 460,000

Unlock all answers

Related textbook solutions

Molecular Cell Biology

Biology: Science for Life with Physiology

Biology

Concepts of Biology

Essentials of Biology

Related questions

Related Documents

BIO 3153 Lecture Notes - Lecture 2: Eosin, Immunofluorescence, Antibody

BIO370Y5 Chapter 2: Microscopy

BIO 1140 Chapter 2.3-3.5: Lecture 2 Reading Notes

Weekly leaderboard