answers

watching

views

31 Jan 2018

in Microbiology LAB :

1. How do you inoculate and streak a culture for isolation?

2. Can you identify colony morphology?

3. What is aerotolerance?

4. What is an example of an anaerobe? An aerobe?

5. In a microscope how do you use, handle, and clean them?

6. How does Gram staining act to stain bacteria?

7. What do the following test for? (biochemical reaction, organism)

answers

watching

122

views

For unlimited access to Homework Help, a Homework+ subscription is required.

You have 0 free answers left.
Get unlimited access to 3.8 million step-by-step answers.

Get unlimited access

Already have an account? Log in

teacherrecoLv10

28 Jul 2023

Unlock all answers

Get 1 free homework help answer.

Already have an account? Log in

You have 0 free answers left.
Get unlimited access to 3.8 million step-by-step answers.

Get unlimited access

Already have an account? Log in

Reid WolffLv2

2 Feb 2018

Already have an account? Log in

Related questions

In a quadrant streak, why do you intersect the previous lines and not the ends of the streak?

Select one:

a. More cell amount variability at ends, usually higher cell population

b. Keep streaks in the middle of the plate

c. Prevent cell death

d. To maintain a mixed culture

e. To count CFUs

How should you hold the inoculating loop?

Select one:

a. Maintain it at an angle at all times

b. In a test tube rack at all times

c. Like a pencil

d. Balanced on top of the tube

e. With dish gloves

Which of the following is used in the spread plate and not the streak plate method?

Select one:

a. Glass spreading rod

b. Flame loop

c. Agar plate

d. Bunsen burner/sterile utensil

e. Starting culture to sample

What would likely happen if the inoculum was not spread adequately over the agar surface?

Select one:

a. Contamination

b. Degradation

c. Sterilization

d. Uneven distribution

e. Cells will not grow

You have a mixed culture of unknown bacteria and want to identify one of the species by several subsequent experiments (needing multiple colonies of a pure culture). You do a quadrant streak and transfer to a sterile liquid medium. What next step makes the most sense?

Select one:

a. Perform a Gram stain

b. Note all colony morphology characteristics

c. Use the spread plate method or new streak plate on an isolated colony of the plate

d. Quantify the cells

e. Repeat using the other two methods of isolation

What would likely occur if the flame loop was not sterilized between streaks on the quadrant streak method?

Select one:

a. Bacteria will not stick to the loop

b. Virus will be introduced

c. Flame loop will be too hot

d. The sample will be contaminated

e. Subsequent streaks would have more colonies

Why is the glass rod in the spread plate method put in alcohol and ignited instead of sterilized like a flame loop?

Select one:

a. Glass oxidizes

b. Glass melts

c. Glass takes longer to cool

d. Glass burns

e. Glass is more sterile

You are working in a food safety lab and were given some samples to evaluate for microbial contamination. The particular sample youâre working with today is a potato purchased at local farmerâs market booth. This farm does not use organic growth techniques. You swabbed the potato and performed a T-streak on MacConkey agar. From this you isolated several different colony morphologies, but one was of particular interest. The colonies were round and very glossy. On MacConkey agar they develop a reddish color.

You amplified one of these colonies for further analysis.

1. You prepare a wet mount and observe the microorganism under high magnification. You find that it has a rod shape and is non-motile.

2. You inoculate a thiolglycolate tube with this organism and incubate it overnight. Results are shown below.

Growth in Thiolglycolate (it is cloudy all the way to the bottom)

3. You inoculate a BD Enterotube with your unknown isolate. The results are shown below.

1 2 3 5 6 7 8 9 11 12

Glucose Lysine Ornithine Adonitol Lactose Arabinose Sorbitol V/P Urea Citrate

Color Yellow Purple Yellow orange yellow yellow yellow red pink blue

Gas? Yes

Note: I left out chambers 4 and 10 since you have no experience reading them and interpreting the results. The organism is negative for Indole, H2S, Dulcitol, and PA.

Case Study 4

Name: ____________________________________

Questions:

1. How would you classify this organism based on oxygen tolerance?

2. What is the scientific name of your microbe?

3. Describe the process you used to come to this result.

4. Is this a microbe that would be expected on produce?

5. Is this microbe a pathogen? Is it an opportunistic pathogen?

ochreworm729

ASEPTIC TECHNIQUE

Introduction Procedure < Include information that the readerwould need to repeat your experimental procedure. Observations andResults Gram stain result: (Experiment 1 and 2) Part I:Contamination Step 6: What color are the traffic lights after capsare removed? Step 7: What color are the traffic lights after capshave been left off and then replaced? Step 9: Appearance of tube:Step 12: Appearance of tube after overnight incubation: Part II:Aseptic Transfer Tube-to-Tube Test Was your aseptic transfersuccessful? (Contamination light and Inoculation light both green)Appearance of tube after overnight incubation: Discussion In theDiscussion section, answer the following questions: Whatprecautions are taken in a "wetlab" to prevent contamination?During the "Contamination" portion of the exercise, why was thecontamination not present when you first placed the phenol redglucose tube in the 37 degree incubator? After your sample had beenincubated for one day, why is the solution turbid? What did thecolor change of the medium indicate? What caused the contaminationof your sample? How successful were you with the aseptic technique?How many times did you need to repeat the experiment until you wereable to successfully inoculate your media without contamination?How important is sterile technique in a "wetlab?" Why? Conclusion< Within a few sentences, provide a concluding statement aboutthe results of your laboratory>

BACTERIAL ANATOMY Introduction Procedure < Includeinformation that the reader would need to repeat your experimentalprocedure. Do not include any observations or results in thissection> Observations and Results Gram stain result: (Experiment1 and 2) Discussion In the Discussion section, answer the followingquestions: Describe the morphology of each bacterium, e.g., cocci,rods, or spiral/curved. Describe the arrangement of the bacteria.Were the bacteria organized as singles, pairs, chains, clusters, oranother type of arrangement? Describe the Gram Stain procedure andhow to interpret the results. How does this apply to your Sample #1and Sample #2? Which stain is responsible for the results that yousee? Relate the results to cell structure. You can also useInternet resources. Make sure that you cite all external datasources, including your textbook. Indicate the terminology that isused for the cell anatomy and arrangement observed for the organismin Sample #1 and Sample #2.