1) What are the two types of termination in prokaryotictranscription?
2) The peptidyl transferase has been determined to come from a23S RNA of the ribosome, making it what type of molecule?
3) It has been determined that the release factors are actuallyproteins that mimic what types of molecules?
1) What are the two types of termination in prokaryotictranscription?
2) The peptidyl transferase has been determined to come from a23S RNA of the ribosome, making it what type of molecule?
3) It has been determined that the release factors are actuallyproteins that mimic what types of molecules?
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Gene ExpressionQuestions
Question 1
#1) Match the following reactions to the correct step of proteinsynthesis by ribosomes.
___ Hydrolysis of GTP and disassembly of the translationcomplex.
___ binding of initiator aminoacyl-tRNA
___ formation of the peptide bond
___ translocation of ribosome
___ Use of 2 mole-equivalents of ATP and attachment of an aminoacid to a transfer RNA molecule
Answer Choices for Number #1
A. initiation step
B. elongation step
C. termination step
D. aminoacyl-tRNA synthetase reaction
Question 2
#2) Mark which of the following are needed for the synthesis ofRNA by RNA polymerase:
___ primer
___ ATP, CTP, GTP, UTP
___ dATP, dCTP, dGTP, dTTP
___ template
Question 3
#3) Mark which of the following are properties of a bacteria(prokaryotic) messenger RNA:
___ | It has a cap at the 5'-end. | |
___ | It has a poly-A tail at the 3'-end. | |
___ | It has a 5'-untranslated region (UTR). | |
___ | It has a 3'-untranslated (UTR) region. |
Question 4
#4) Match the following RNA molecules to their respectivefunctions in the cell.
___ transfer RNA
___ ribosomal RNA
___ messenger RNA
___ small nuclear RNA (snRNA)
Answer Choices for Number #4
A. This RNA serves as the template for protein synthesis.
B. This molecule is a molecular component of ribosomes.
C. This molecule is a component of spliceosomes and serves torecognize the exon-intron junctions
D. This RNA molecule is delivers the amino acid to the ribosome andit recognizes the appropriate codons with its anticodon loop.