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22 Apr 2018

Which process is faster or slower in non-homologous pair and homolgous recombination?

Please help me I have that the nonhomologous repair is the slower and longer than the recombination repair is this correct please help me out.

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Keith Leannon
Keith LeannonLv2
25 Apr 2018

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Related questions

Trying to work out and reason through this practice question tohelp me on my test.

"Briefly explain why non-homologous end joining is overwhelmingfavored over homologous recombination repair for fixing doublestrand DNA breaks in mammalian cells in G1. Why is this biasreversed during G2?"

So I reckon that non-homologous end joining is favored in G1because although original base pairing is lost and chewed back byexonuclease before the DNA is re-ligated, the loss of base pairsisn't critical due to the imminent S phase, which will ensure theDNA is further re-synthesized, thus mitigating the effect of lostbase pairs.
However, for G2, nonhomologous recomb. is favored because in thismethod base pairs are re-added before getting re-ligated, ensuringno bases are lost. This is critical because the next phase ismitosis, and all of the DNA must be synthesized to this point; anyloss of DNA before mitosis would probably lead to mutations whenthe cell eventually divides.

Is my reasoning accurate? Please chime in anddiscuss!

12. During homologous recombination, what events are required for formation of the heteroduplex?

13. Assume that homologous recombination occurs across a region of DNA containing a wild-type and mutant allele of a particular gene. There is a single base pair change in the mutant allele as follows:

wild-type = 5’…TTGGGTA… à 5’…TTGCGTA… = mutant allele, such that the resulting dsDNA is as follows:

5’…TTGGGTA…

3’…AACGCAT…

What would be the genotype (wild-type or mutant) if base excision repair occurs on the TOP strand?

14. What term describes the process occurring above?

Please help with some epgenetics! I got partial credite for the answers I put but I'm not sure which ones I got wrong!

1) Match the repair pathway to the type of damage it most commonly repairs.

a. Non-homologous end joining

b. Nucleotide excision repair

c. Mismatch repair

d. homologous recombination

e. photolyse

Choices: double strand breaks, UV-induced photodamage, replication error, spontaneous depurination, 5-methyl cytosine

My answer: a. 5-methyl cytosine, b. UV-induced photodamage, c. replication error, d. double strand breaks, e. spontaneous depurination

2) Match the human diseas to the repair patheay that is deficient that disease. Answers may be used more than once.

a. Hereditary polypsis colorectal cancer

b. Xeroderma pigmentosum

c. Ataxia telangiectasia

d. Fanconi Anemia

Choices: mismatch repair, nucleotide excision repair, double strand break repair, base excision repair, photolyse

My answer: a. mismatch repair, b. nucleotide excision repair, c. mismatch repair, d. double strand break repair

3) ____ (a) is a histone chaperone that is recruited by the PCNA and binds to newly synthesized _____(b).

______ (c) is a histone chaperone that acts during the replication-independent assembly pathway, when _____ (d) is incorporated into chromatin.

______(e) is a thrid chaperone that is recruited to replication forks by the replicative helicase.

My answers: a. CAF1, b. H3 and H4, c. H3.3, d. nucleosomes, e. ASF

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