Differential centrifugation separates cell components according to
Mass
Sedimentation rate
Density
Charge
Density gradient centrifugation separates cell components according to
Density
Sedimentation rate
Mass
Charge
If you are presented with a graph with many fractions on the x-axis, or a western blot where the lanes were from many fractions, what method was used?
Affinity purification
Western blot
Density gradient centrifugation
Differential centrifugation
What is the principle behind denaturing protein gel electrophoresis?
Proteins are separated according to type of amino acid R groups
Proteins are separated according to charge
Proteins are separated according to size
In denaturing gel electrophoresis, proteins that run farther down the gel are:
Smaller than those at the top of the gel
Larger than those at the top of the gel
What is a western/immunoblot?
All proteins are stained with a general protein stain
Specific proteins are identified by antibodies
Specific proteins are purified and detected by a general protein stain
What is the principle behind affinity purification?
Specific molecules are removed from solution by a high affinity interaction on a solid support
Specific molecules have an exclusive sedimentation rate that be exploited with differential centrifugation
Specific molecules have an exclusive size that can be exploited with SDS-PAGE
Which of the following is quantification of variance in an experiment?
Median
Error bars
Mean
Differential centrifugation separates cell components according to
| Mass | |
| Sedimentation rate |
| Density | |
| Charge |
Density gradient centrifugation separates cell components according to
| Density | |
| Sedimentation rate |
| Mass | |
| Charge |
If you are presented with a graph with many fractions on the x-axis, or a western blot where the lanes were from many fractions, what method was used?
| Affinity purification | |
| Western blot |
| Density gradient centrifugation | |
| Differential centrifugation |
What is the principle behind denaturing protein gel electrophoresis?
| Proteins are separated according to type of amino acid R groups | |
| Proteins are separated according to charge |
| Proteins are separated according to size |
In denaturing gel electrophoresis, proteins that run farther down the gel are:
| Smaller than those at the top of the gel | |
| Larger than those at the top of the gel |
What is a western/immunoblot?
| All proteins are stained with a general protein stain | |
| Specific proteins are identified by antibodies |
| Specific proteins are purified and detected by a general protein stain |
What is the principle behind affinity purification?
| Specific molecules are removed from solution by a high affinity interaction on a solid support | |
| Specific molecules have an exclusive sedimentation rate that be exploited with differential centrifugation |
| Specific molecules have an exclusive size that can be exploited with SDS-PAGE |
Which of the following is quantification of variance in an experiment?
| Median | |
| Error bars |
| Mean |
