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6 Jul 2019
3) You have the following stock solutions and solid chemicals in your lab:
-2.0 M NaCl
-0.50 M Tris buffer, pH 8.0
-20 mg/ml Bovine serum albumin (BSA)
--MgCl2 (solid, anhydrous) MW = 95.211 g/mole (95 % pure)
-Glucose (solid, anhydrous)
How would you make 250 ml of a solution containing all of the following:
0.1M NaCl,
0.02 M Tris, pH 8.0,
0.5 mg/ml BSA,
25 mM MgCl2,
150 mg% glucose.
List the volumes of each stock solution and the weights of each solid. Include calculations for each component.
3) You have the following stock solutions and solid chemicals in your lab:
-2.0 M NaCl
-0.50 M Tris buffer, pH 8.0
-20 mg/ml Bovine serum albumin (BSA)
--MgCl2 (solid, anhydrous) MW = 95.211 g/mole (95 % pure)
-Glucose (solid, anhydrous)
How would you make 250 ml of a solution containing all of the following:
0.1M NaCl,
0.02 M Tris, pH 8.0,
0.5 mg/ml BSA,
25 mM MgCl2,
150 mg% glucose.
List the volumes of each stock solution and the weights of each solid. Include calculations for each component.
Elin HesselLv2
6 Jul 2019
