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15 Aug 2018

10. Covalent post-translational modifications can alter the structure and function of proteins, allowing them to operate in specific regions of the cell, perform specific functions, or interact with specific proteins. In lecture, we discussed several types of covalent post-translational modifications and their roles in protein localization, folding, or function. Choose one example from each of the following categories and describe the modification and, generally, what it does the localization/function of the protein: glycosylation, lipidation, polypeptide attachment.

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Jarrod RobelLv2

16 Aug 2018

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2260 A&P 1 Lecture - Unit 1

Beware! ANYTHING within the assigned readings or discussed in lecture is fair game whether it is part of this list or not!!

What is the study of Anatomy? Provide an example of an anatomical study.

What is the study of Physiology? Provide an example of a physiological study.

List the characteristics of living things and briefly discuss them.

List the levels of organization from smallest to largest. Provide a specific example for each level.

Compare/Contrast sections and planes.

What are the distinguishing characteristics of the anatomical position?

Practice placing a section through a location in the body and then determine what structures would be made visible by that section in that location. For example if a coronal (frontal) section were made in the thoracic region (would be able to see both lungs, the heart, depending on the exact location of the cut, maybe the esophagus and /or the trachea)

Practice the directional terms using them to describe the locational relationship between two body regions (use the technical terms for those body regions, (i.e. if the original post used __ is superior to blank, the opposite would be ___ is inferior to __).

Distinguish between the nine abdominal regions and four quadrants and indicate what major organ(s) would be found deep to that region or quadrant.

Why is it important to study the language of anatomy and physiology if one is going into the medical field?

Define homeostasis. What thing (or things) in our body maintains homeostasis? And what regulates those "things"?

What is the likely result if homeostasis is not maintained?

13. What are the components of feedback mechanisms? What is the function of each component?

14. Discuss negative feedback mechanisms. Give a specific example of one.

Discuss positive feedback mechanisms. Give a specific example of one.

Discuss the levels of organization, list the various levels and provide a specific example of each level.

What is matter?

What are elements? What are the four most common elements of the human body?

Distinguish between atoms, and molecules. Provide an example of each.

What information is provided by the molecular formula? For example what can be said of the formula C₆H₁₂O_6, the molecular formula for glucose?

Briefly distinguish between organic and inorganic molecules. Recall that MY preferred defining quality differs from what the text says!

Consider/review the important inorganic molecules discussed in chp 2 and describe their importance in the human body.

Distinguish between acids and bases. Which of these will have relatively more free H+ ions?

What is the pH range of an acid? Of a base? What would be the pH value of a solution where the number of H+ ions = OH- ions?

What are the states of acidosis and alkalosis with reference to blood pH values?

Consider/review the important organic molecules discussed in chp 2 and describe their general importance in the human body.

Distinguish between solvent and solute.

Discuss the term osmolarity.

Consider/review the four types of lipids and discuss their individual function in the human body. Lipids are not composed of monomers but they do have âbuilding blocksâ, what are these building blocks?

What are the "building blocks" or monomers (looking for a group or class name) of a carbohydrate polymer? Name the five (5) types of carbohydrate monomers.

When two of these carbohydrate monomers are bonded together what "class" of carbohydrate is formed? What are the types of this "class"? What is the source (food) for each of these?

When three or more of these monomers are bonded together they form which "class" of carbohydrate? Which one of these stores excess carbs in animals (mainly in the liver)? Which one stores excess carbs in plants? And which one is indigestible by the human gut, but is important because it provides "bulk" in the form of fiber and helps keep fecal matter moving in the intestines?

What is the monomer of nucleic acids?

Briefly describe deoxyribonucleic acid including its general function.

Briefly describe ribonucleic acid including its general function.

What are the monomers of proteins? What kind of bond holds the monomers together?

Distinguish between oligopeptides and polypeptides.

Choose TWO general functions of proteins based on the globular vs fibrous classification of proteins, the class of protein that performs that function and a specific example for each.

What events or environments denature a protein? What has happened if a protein is denatured? What happens to its function if it is denatured?

What class of organic molecules are enzymes and what do they do in the body? How do they do their function? In other words, what is their mechanism of action/what do they change to do their job?

What are enzyme co-factors and what is their role in enzyme action?

How are enzymes regulated?

I JUST NEED SIMPLE/BASIC ANSWERS NOT LONG ANSWERS

1. Explain how life can be viewed at different levels of biological complexity.

2. Explain how researchers study biology at different levels, ranging from molecules to ecosystems.

3. Distinguish between discovery-based science and hypothesis testing, and describe the steps of the scientific method.

4. Tell whether a given topic or research study is about biology. Tell whether that study is discovery-based or hypothesis testing, or both.

5. If presented with a research study, propose what the Research Question was.

6. If presented with an example, tell whether a statement is a hypothesis, theory or law and why you think so.

Describe the general structure of atoms and their constituent particles.

Relate atomic structure to the periodic table of the elements. List the elements that make up most of the mass of all living organisms.

Explain how a single element may exist in more than one form, called isotopes, and how certain isotopes have importance in human medicine.

Compare and contrast the types of atomic interactions (bonds) that lead to the formation of molecules.

Tell which atoms and how many of each make up a given molecule (formula or diagram).

For a bond in a given molecule, tell whether the bond is covalent, and if it is, tell whether it is polar or nonpolar and why you think so.

Explain the concept of electronegativity and how it contributes to the formation of polar and nonpolar covalent bonds. Tell whether a given atom is electronegative or not, and why you think so. Label partial charges in a polar bond.

Draw five water molecules and label the atoms and bonds within and between the 5 molecules.

Describe how hydrogen bonding determines many properties of water.

List the properties of water that make it a valuable solvent, and distinguish between hydrophilic and hydrophobic substances.

Explain how water has the ability to ionize into hydroxide ions (OH^â) and into hydrogen ions (H⁺), and how the H⁺ concentration is expressed as a solution's pH.

Explain the properties of carbon that make it the chemical basis of all life.

Describe the variety and chemical characteristics of common functional groups of organic compounds.

Diagram how small molecules may be assembled into larger ones by dehydration reactions and how hydrolysis reactions can reverse this process.

List the four major classes of organic molecules and macromolecules found in living organisms

If shown a molecule, tell whether it is organic or not and how you know.

If shown an organic molecule, tell whether it is an amino acid, nucleotide, fatty acid, steroid, monosaccharide or disaccharide and how you know.

Distinguish among different forms of carbohydrate molecules, including monosaccharides, disaccharides, and polysaccharides.

Relate the functions of plant and animal polysaccharides to their structure.

List the several different classes of lipid molecules important in living organisms.

Diagram the structure of a triglyceride and explain how it is affected by the presence of saturated and unsaturated fatty acids. Explain why some fats are solid at room temperature, and others are liquid.

Discuss how fats function as energy-storage molecules.

Apply knowledge of the structure of phospholipids to the formation of cellular membranes.

Describe the chemical nature of steroids and give an example of their biological importance.

Give examples of the general types of functions that are carried out in cells by different types of proteins.

Explain how amino acids are joined to form a polypeptide, and distinguish between a polypeptide and protein.

Describe the levels of protein structure and the factors that determine them. If shown a protein diagram, label parts that represent its levels of structure. Tell what kind of research techniques are used to determine protein structure.

Outline the bonding forces important in determining protein shape and function.

Explain what domains are and their importance in proteins.

Describe the three components of nucleotides.

Distinguish between the structures of DNA and RNA.

Tell how certain bases pair with others in DNA and RNA.

Discuss the 4 major categories of organic macromolecules found in living things, including structure, function and location of each.

Compare and contrast the general structural features of prokaryotic and eukaryotic cells.

Outline the differences in complexity among bacteria, animal, and plant cells.

Describe how a eukaryotic cell can be viewed as four interacting systems: the nucleus, cytosol, endomembrane system, and semiautonomous organelles.

Explain how the proteome underlies the structure and function of cells.

Analyze how cell size and shape affect the surface area/volume ratio.

Identify the location of the cytosol in a eukaryotic cell and list its general functions.

Describe the three types of protein filaments that make up the cytoskeleton. Identify which underlie cilia, flagella, centrioles, the nuclear lamina, and the brush border. Tell which are readily assembled and disassembled. Tell which are involved in various cell shape changes or movements.

Explain how motor proteins interact with microtubules or actin filaments to promote cellular movement.

Describe the structure and organization of the cell nucleus. Describe the structure and function of nuclear pores.

Outline the structures and general functions of the components of the endomembrane system.

Distinguish between the rough endoplasmic reticulum and the smooth endoplasmic reticulum.

Identify three important functions of the plasma membrane.

Tell why mitochondria and chloroplasts are called semi-autonomous organelles.

Discuss the evidence for the endosymbiosis theory.

Outline the structures and general functions of mitochondria and chloroplasts.

Explain how proteins are moved via vesicles through the endomembrane system.

If you are told about the function of a specific protein, tell where and in what type of cell that protein might be found.

Sketch and label a âtypicalâ animal cell and a real animal cell such as a red blood cell or a muscle cell. Tell how it might be different than the âtypicalâ cell in terms of its organelles and shape.

Sketch and label a âtypicalâ plant cell and a real plant cell such as a root or leaf cell. Tell how it might be different than the âtypicalâ cell in terms of its organelles and shape.

Describe the fluid-mosaic model of membrane structure. Tell why membranes are described as âfluid mosaicsâ.

Draw 10 phospholipid molecules as they would be arranged in a bilayer. Label the hydrophobic and hydrophilic parts.

61. Name all the places in a cell where membranes are found.

62. Describe at least three different categories of membrane proteins and tell what they do.

63. Describe what determines how fluid membranes are.

64. Explain how lipids are synthesized at the ER membrane.

65. Explain how transmembrane proteins are inserted into the ER membrane.

66. Compare and contrast diffusion, facilitated diffusion, passive transport, and active transport.

67. Explain the process of osmosis and how it affects cell structure.

68. Outline the functional differences between channels and transporters. Compare and contrast uniporters, symporters, and antiporters.

69. Explain the difference between primary active transport and secondary active transport.

70. Describe the structure and function of the sodium-potassium pump in animal cells and a similar pump in plants and prokaryotes.

71. Describe exocytosis and endocytosis and tell what molecules are moved across the membrane by these.

Discuss membrane transport, including its function and various forms and why all membranes have each form.

73. If a given cellular process is described, tell which form of transport might be involved.

List the different categories of animal tissue, providing general functions and specific examples of each.

Name the various organ systems found in many animals, list the components of each, and describe their general functions.

bluebeaver798

__________ are fairly small organelles that provide a safe place within the cell to carry out certain biochemical reactions that generate harmful, highly reactive oxygen species. These chemicals are both generated and broken down in the same location.

Lysosomes

Endosomes

Peroxisomes

Nucleosomes

Biologists cannot possibly study all living species. Instead, they try to understand cell behavior by studying a select subset of them. Which of the following characteristics are useful in an organism chosen for use as a model in laboratory studies?

rapid rate of reproduction

all of the above

ability to grow under controlled conditions

amenability to genetic manipulation

By definition, prokaryotic cells do not possess __________.

ribosomes.

a nucleus.

membrane bilayers.

replication machinery.

Cell biologists employ targeted fluorescent dyes or modified fluorescent proteins in both standard fluorescence microscopy and confocal microscopy to observe specific details in the cell. Even though fluorescence permits better visualization, the resolving power is essentially the same as that of a standard light microscope because the resolving power of a microscope is limited by the __________ of light.

intensity

absorption

wavelength

filtering

Choose the phrase that best completes this sentence: Microtubules ____________ and are required to pull duplicated chromosomes to opposite poles of dividing cells.

generate contractile forces

can rapidly reorganize

are found in especially large numbers in muscle cells

are intermediate in thickness

Despite the differences between eukaryotic and prokaryotic cells, prokaryotes have proteins that are distantly related to eukaryotic actin filaments and microtubules. What is likely to be the most ancient function of the cytoskeleton?

cell division

cell motility

vesicle transport

membrane support

DNA and RNA are different types of nucleic acid polymer. Which of the following is true of DNA but not true of RNA?

It contains thymine.

It has 5'-to-3' directionality.

It is single-stranded.

It contains uracil.

Double covalent bonds are both shorter and stronger than single covalent bonds, but they also limit the geometry of the molecule because they ____________________.

prevent additional bonds from being formed with the bonded atoms.

limit the rotation of the bonded atoms.

create a new arrangement of electron shells.

change the reactivity of the bonded atoms.

Drosophila melanogaster is a/an __________. This type of animal is the most abundant of all animal species, making it an appropriate choice as an experimental model.

mammal

amphibian

bird

insect

10.

If the isotope 32S has 16 protons and 16 neutrons, how many protons, neutrons, and electrons will the isotope 35S have, respectively?

16, 19, 17

16, 19, 15

16, 19, 16

16, 20, 15

11.

Living systems are incredibly diverse in size, shape, environment, and behavior. It is estimated that there are between 10 million and 100 million different species. Despite this wide variety of organisms, it remains difficult to define what it means to say something is alive. Which of the following can be described as the smallest living unit?

cell

organelle

protein

DNA

12.

Macromolecules in the cell can often interact transiently as a result of noncovalent interactions. These weak interactions also produce stable, highly specific interactions between molecules. Which of the factors below is the most significant in determining whether the interaction will be transient or stable?

the concentration of each molecule

the size of each molecule

surface complementarity between molecules

the rate of synthesis

13.

Many of the mechanisms that cells use for maintenance and reproduction were first studied at the molecular level in bacteria. Which bacterial species had a central role in advancing the field of molecular biology?

E. coli

S. pombe

C. elegans

D. melanogaster

14.

Many types of cells have stores of lipids in their cytoplasm, usually seen as fat droplets. What is the lipid most commonly found in these droplets?

cholesterol

isoprene

triacylglycerol

palmitic acid

15.

Mitochondria contain their own genome, are able to duplicate, and actually divide on a different time line from the rest of the cell. Nevertheless, mitochondria cannot function for long when isolated from the cell because they are __________________.

anaerobes.

viruses.

parasites.

endosymbionts.

16.

Mitochondria perform cellular respiration, a process that uses oxygen, generates carbon dioxide, and produces chemical energy for the cell. Which answer below indicates a correct pairing of material âburnedâ and the form of energy produced during cellular respiration?

17.

Polar covalent bonds are formed when the electrons in the bond are not shared equally between the two nuclei. Which one of these molecules contains polar bonds?