Lecture : BIO-226 Exam 4 Summary (1)

1.Whati is Genetic Technology?What is bacterial transformation?
What is recombinant DNA technology?What is a vector?What is found in a typical plasmid?How can DNA sequenced use the dideoxy chain termination method? Describe the polymerase chain reaction (PCR). What are some advantages and limitations of this procedure?What is a cDNA? How are cDNAs and DNA microarrays used to study gene expression?Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology,Explain how the creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA moleculOutline the procedures for cloning a eukaryotic gene in a bacterial plasmid.Describe techniques that allow identification of recombinant cells that have taken up a gene of interest.How can you use the lacZ gene to facilitate cloning ?Distinguish between genomic and cDNA libraries.Define genomics and proteomics.Explain how gel electrophoresis is used to analyze nucleic acids and to distinguish between two alleles of a gene.How does dideoxy sequencing work? What is the significance of using dideoxy nucleotides?What are BACs, YACs, and contigs? What are some practical applications of DNA technology?What are the ways in which organisms can be genetically modified?

The strongest direct evidence that DNA is the genetic material comes from what contemporary methodology?

I know it is recombinant DNA technology, but I am having a hard time understanding the reason why this is.

Understand the usefulness of bacterial plasmids.

Benefits provided so far from DNA technology and genetic engineering?

Benefits provided from the study of Genomics?

Understand how restriction enzymes work.

To what does ‘recombinant DNA’ refer?

Genome, intron vs exon (what is the function of the exons?).

How is DNA extracted?

How does gel electrophoresis work?

PCR (polymerase chain reaction) is used for what purposes?

Molecular cloning vs reproductive cloning.

Genetically modified vs transgenic.

What happened to ‘Dolly’ (the first cloned mammal)?

Have scientists tried to bring back any extinct species via cloning? If so, which ones?