BIO 325 Lecture Notes - Lecture 7: Replica Plating, Wild Type, Base Pair

• School: UT-Austin
• Department: Biology
• Course: BIO 325
• Professor: A Lloyd

Document Summary

Classification of mutations by effect on dna molecule. Substitution - base is replaced by one of the other three bases. Deletion - block of one or more dna pairs is lost. Insertion - block of one or more dna pairs is added. Inversion 180 degree rotation of pieces of dna. Reciprocal translocation - parts of no homologous chromosomes change places. Chromosomal rearrangements affect many genes at one time. Spontaneous mutations influencing phenotype occur at a very low rate. If mutation is so rare, how did mendel get pea mutants? (7: he already had those mutations accumulated. General observations of mutation rates: mutations affecting phenotype occur very rarely, different genes mutate at different rates. Are mutations spontaneous or induced: most mutations are spontaneous, they are not induced by selection conditions. Luria and delbruck experiments - simple way to tell if mutations are spontaneous or if they are induced by condition or a mutagenic agent.