BIO SCI 99 Lecture Notes - Lecture 10: Repressor Lexa, Blastocyst, Somatic Cell Nuclear Transfer
1 Sep 2016
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Document Summary
Bacteria only have 1 kind of rna pol. Determines how efficient the polk binds to promoter region. Lac promoter expresses lac operon which encodes protein that metabolizes lactose. Experiments showed that mutations that bring lac closer to consensus = higher levels of expression. Somatic cell nuclear transfer- makes an embryonic stem cell from adult cell by reprogramming transcription of composite cell. Able to make specific cell types with specific transcription factors. Totipotent- ball of cells after a few divisions. More division makes blastocyst which becomes the placenta. Inner layers make germ layers (ectoderm, mesoderm, and endoderm) which are pluripotent- can become any cells and differentiated into any type of tissue. Multipotent- (adult stem cells) can become different type of blood cells or regenerate bone. They have a niche- microenvironment that promotes stem cell maintenance. Hard to use for medicine bc hard to regenerate, limited, and hard to isolate. Work with protein or rna product of gene.
