BIO SCI 97 Lecture Notes - Lecture 1: Polymerase Chain Reaction, Dna Profiling, Phosphodiester Bond

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11 Sep 2017

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You can tell the directionality by looking at the free phosphorus of hydroxyl group. The difference between dna and rna is that. B/c of hydrogen bonds that bond between the base pairs. A = t (2 h-bonds) and g c (3 h-bonds) A = t base pairs are less stable than g c (cid:915)(cid:914)(cid:932)e p(cid:914)i(cid:931)(cid:932) (cid:915)/(cid:916) a = t have less h-bonds. Not a self-assembly process since the base pairs require enzymes. Crick found that dna was a double helix. Watson was the one that found the h-bonds between nucleotide base-pairs. The 2 strands split open, then is copied. The first cycle: each new dna is made up of 1 parent strand, 1 daughter strand. Nucleotides being added into a chain is called polymerization. Phosphodiester bond is between the sugar phosphate backbone of each nucleotide. Triphosphate (atp) attacks the oh and the o- bonds to the oh forms. Hydrolysis (formation of h2o) is a spontaneous reaction.

Related Questions

Relate the following columns:

A. In the complementary strands of DNA are exposed to the aqueous environment.	( ) Bridges of H.
B. They are in the hydrophobic interior of the DNA double helix.	( ) 2-DNA.
C. They are van der Waals interactions between pairs of stacked bases.	( ) Sugar-phosphate skeletons.
D. They are an important stabilizing force between base pairs.	( ) Purines and pyrimidines.
E. It's the approximate conformation found in the cells.	( ) A-DNA.
F. It's favored when the DNA is dehydrated:	( ) Stacking interactions.
G. It's favored in certain sequences rich in G/C	( ) B-DNA.
H. The protein machinery that carries out DNA polymerization.	( ) Okazaki fragments.
I. Short fragments of DNA strand.	( ) DNA Helicase.
J. Just with DNA-PolIII and other proteins is a part of the replisome.	( ) Primosome.
K. Synthesize the RNA primers.	( ) Primase.
L. Unroll the two strands of the parent double helix.	( ) SSB.
M. It joins the double strand and prevents it from bending back.	( ) Replisome.

1.Which of the following is not true about DNA? a) it is found both in the nucleus and the cytoplasm b)it is double stranded and the standards are connected by hydrogen bonds between the base pairs c) A purine base always pairs with a pyrimidine base to make up the DNA code d) it is the control center for both cell activity and heredity 2.Which of the following represents accurate base pairing in DNA ?a) Adenine to Adenine b)Adenine to Uracil c)Thymine to Cytosine d)Adenine to Thymine 3. Purines and Pyrimidines are found a) DNA only b)RNA only c) A and B d)In proteins 4.The bond that forms between carbohydrates is called a)peptide bond b) ester bond c) glycoside bond d)never ending bone 5. Which of the following does not dissolve readily in water? a)proteins b) amono acids c) steroids d) carbohydrates 6. The following arrangement of amino acids represents what level of structure ? ABACABDD a)Primary b)Secondary c)Tertiary d)Quaternary

cerisedog238

1. Which of the following are characteristics of theWatson-Crick

DNA double helix? (there are more than one answer)

a. The two polynucleotide chains are coiled about a common

axis.

b. Hydrogen bonds between A and C and between G and T hold

the two chains together.

c. The purines and pyrimidines are on the inside of the

helix and the phosphodiester backbones are on the

outside.

d. Base composition analyses of DNA duplexes isolated from

many organisms have shown that the amounts of A and T are

equal as are the amounts of G and C.

e. The sequence in one strand of the helix varies

independently of that in the order strand.

2. Which of the following statements about reversetranscriptase

are correct (there are more than one answer,5 points)

a. Reverse transcriptase uses DNA as a template tosynthesize

DNA.

b. Reverse transcriptase uses RNA as a template tosynthesize

RNA.

c. Reverse transcriptase uses DNA as a template tosynthesize

RNA.

d. Reverse transcriptase is found in the virions of all RNA

tumor viruses.

e. Reverse transcriptase hydrolyzes RNA in DNA-RNA hybrid

duplexes.

3. Several dozen oncogenes are known to code for receptortyrosine

kinases. Many of these oncogenes produce mutant receptors,

whose tyrosine kinase activity is permenantly activated,this

is called,(5 points)

a. Autophosphorylation

b. Constitutively active

c. Growth factor dependency

d. a,b,c

e. None of the above

amethysttiger979

BIO SCI 97 Lecture Notes - Lecture 1: Polymerase Chain Reaction, Dna Profiling, Phosphodiester Bond

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