BIO 198 Lecture Notes - Lecture 35: Thymine, Southern Blot, Blastocyst
30 Dec 2016
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In worms, dna is often inserted as a concatemer (multiple tandem copies of the inserted gene) and. Dna can enter the nucleus and become integrated into the genome) how would we know it is incorporated by looking at complementation for that phenotype and then look for expression of reporter genes. You can put sequences into the worm but cannot control how many copies and where it goes (and may not be expressed at normal levels due to position effect variegation). If in inject into developing embryo the fly itself may not have the rosy+ phenotype and must wait till next generation. Mice (there are two different ways to make transgenic mice): If you are injecting the dna at a multicell stage, you will have multiple insertions. Es-b/b m/m (wt embryonic stem cell from a brown mouse b = brown and b = black) In mammalian cells we have lots of introns/exons.
