LIFESCI 2A03 Lecture Notes - Lecture 6: Cancer Stem Cell, Cell Theory, Immunodeficiency
23 Feb 2017
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Gene bam expressed in cytoblast and not germline stem cells. Cytoblast is like nucleus of the cell. Use bam as a marker, make a dna construct that has bam promoter. Promoter part of dna that binds transcription factors, and controls the expression of gene. Since bam is expressed in cytoblast, bam can be used as a marker for cytoblasts. Bam is transcribed in gsc but not cytoblasts. A transposon (mobile genetic element) present in drosophola. A transposon is something that can change its position within a genome. Can change its position within genome by cut and paste mechanism. Helper plasmid contains: dna coding transposase enzyme. Transposase recognition element reporter gene e. g. red-eye marker the product of the white gene. Transposase is found in all organisms, but p element is only in drosophila. It is a jumper gene that jumps around in the genome, they exploited this ability of the gene to the genome.
