BIO 110 Lecture 15: BIO 110

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5 Oct 2016
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Simultaneous transcription and translation only happens in prokaryotes (no nucleus) An early proposal for translation & a genetic code. Dna acts directly as a template for protein assembly. Amino acids fit in the cavity between 4 bases in the major groove of the double helix. Various combinations of bases form a distinct cavities into which the side chains of amino acids might fit. When all the amino acids were lined up in the correct order along the groove, an enzyme would come along to polymerize them. E. coli extract (without mrna) + specific mrna new protein. Polynucleotide phosophorylase 3" to 5" exoribonuclease activity. Give ribosomes a rna template - see what amino acids are in the protein it makes. Its more complicated since precise templates could not be made. Another experiment to confirm and finish the genetic code. Basic experiment: give ribosomes a 3 bp rna template.

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