01:119:115 Lecture Notes - Lecture 17: Taq Polymerase, Polyacrylamide Gel Electrophoresis, Dna Ligase

• School: Rutgers University
• Department: Biological Science
• Course: 01:119:115
• Professor: Daniel Stern Cardinale

Document Summary

Dna technology: techniques for sequencing and manipulating dna. Base pairing of one strand of nucleic acid to complementary strand. Dna sequencing: uses the principle of complementary base pairing to determine a gene"s sequence. Base pairing sequence: automated by sequencing machines, dideoxyribonucleotide (=dideoxy) chain termination sequencing. Allows one to determine sequence of dna fragments ~ 1000 base pairs (bp) Name based on the sugars that are present. Dna - (c. 3. b. 1) deoxyribose (c. 3. b. 1. a) lacks 2" oh (c. 3. b. 2) dideoxy (c. 3. b. 2. a) lacks 2" oh group, also lacks 3"oh. Machine (c. 4. b. 1) synthesis of new strands (c. 4. b. 1. a) begin at 3" end of primer (c. 4. b. 1. b) continues until a dideoxyribonucleotide is inserted/added. Restriction enzymes (restriction endonucleases: produced by bacteria. Cut up and destroy foreign dna: recognize and cut restriction site/foreign dna. Bacterial dna is protected from its own restrictive enzymes because methylation of a"s or c"s within site: ex: hind iii. 5" - a - a - g - c - t - t - 3".