01:119:115 Lecture Notes - Lecture 17: Molecular Cloning, Recombinant Dna, Dna Ligase
13 Jul 2016
School
Department
Course
Professor
Document Summary
Dna technology techniques for sequencing and manipulating dna. Biotechnology manipulation of organisms or their components to make useful products. Nucleic acid hybridization the base pairing of one strand of nucleic acid to the complementary sequence on another strand. Genetic engineering direct manipulation of genes for practical purposes. Use sequencing complete genomes evolutionary relationships. Use principle of complementary base pairing to determine gene"s complete sequence of nucleotides. Dideoxy or chain termination sequencing 1st automated procedure up to 1000 bp sequencing. Next-generation sequencing most recent one does not rely on chain termination. Up to 100"s of millions bp sequence: dideoxy chain termination sequencing. Rna ribose but dna deoxyribose = lacks 2" oh. Dideoxyribose also lacks 3" oh group (to which next nucleotide would normally link) Dna to be sequenced denatured into single strand (figure 20. 2) In sequencing machine synthesis starts at 3" end of primer. Continues until a dideoxyribonucleotide inserted (at random) Prevents further elongation of strand (b/c no free 3" oh)
