BIOS 10161 Lecture Notes - Lecture 4: Lightning, Thomas Cech, Sidney Altman
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You decide to identify the CFTR mutation by analyzing the genomic DNA of your patients compared to healthy individuals. You specifically are looking to see whether a specific 3' gene truncation has occurred in the patients. You will determine this using hybridization techniques with samples from healthy and CF patients. Which of the following will allow you to accomplish this?
Using an RNA probe complementary to the region not removed by the truncation. | |
Using an RNA probe complementary to the region removed by the truncation. | |
Using an DNA probe complementary to the region not removed by the truncation. | |
Using an DNA probe complementary to the region removed by the truncation. |
To conduct the hybridization experiment, you are trying to decide between using a DNA or RNA probe. Which would be ideal to use and why?
As both are composed of nucleic acids, using either would result in identical results. | |
An RNA probe because RNA has uracil bases. | |
An RNA probe because it could also be used in a translation experiment. | |
A DNA probe because it is more stable than RNA. | |
A DNA probe because RNA cannot bind to DNA. |
One step of the Hershey/Chase experiment involved blending the virus/cell mixture before centrifugation and probing the pellet for radioactivity. Why was the blending step necessary?
To collect the bacteria at the bottom of the tube. | |
To break open the bacteria to release the genome. | |
To separate the bacteria from the bacteriophages. | |
To be able to detect the radioactivity. |
Imagine Hershey/Chase had used an RNA virus (genome composed of RNA) instead of a DNA virus in their experiment. Would radioactivity still have been found in the pellet?
No, because only DNA can be labeled with radioactivity. | |
No, because the RNA genome would not enter the bacteria upon infection. | |
No, because while DNA and RNA nucleotides are similar, they are not identical. | |
Yes, because DNA and RNA nucleotides are similar. | |
Yes, because genome in any form (DNA, RNA, protein) would be labeled similarly. |
The human genome consists mostly of non-coding DNA. Which of the following are benefits of this?
Random DNA mutations generally won't affect RNA and protein function. | |
It is faster to duplicate the genome when these are present. | |
The existence of introns can lead to multiple variations of proteins encoded by a single gene. | |
It is unlikely transposons would exist in the genome if there was too much protein coding DNA. |
Explain the 5â to 3â directionality of a DNA stand.
It is due to the fact that the free 5â carbon is on one end and the free 3â carbon is on the other | |
It is due to the fact that new nucleotide are added to the 5â carbon of the previous nucleotide | |
It is due to the fact that there are 3 phosphate groups attached to the 5â carbon | |
It is due to the fact the complementary strand is 3â to 5â | |
More than one of the above explain the 5â to 3â directionality |
You accidentally add a mutant dNTP (which has an H instead of an OH connected to the 3â carbon) to a reaction where DNA is being replicated. Which of the following is true of this mutant dNTP?
It can be incorporated into DNA strand but cannot form a phosphodiester bond with an incoming wild type dNTP | |
It can be incorporated into a DNA strand but cannot base pair with a complementary nucleotide | |
It can be incorporated into a DNA strand and can form a phosphodiester bond with an incoming dNTP, but only if it is another mutant dNTP | |
It cannot be incorporated into a DNA strand. |
Why does DNA polymerase utilize an RNA primer?
DNA polymerase is unable to initiate strand synthesis but RNA polymerase can | |
DNA polymerase can only add a dNTP to an rNTP | |
DNA synthesis proceeds in the 3â to 5â when initiating strand synthesis | |
Chromosomal DNA contains interspersed RNA fragments | |
The RNA primer increases stability of the newly synthesized strand |
Question 401 pts
Kinetic energy is the stored energy that can be used for motion.
True |
False |
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Question 411 pts
When an electron is transferred from one atom to another, and the two atoms are then electrically attracted to one another, a(n) ________________ bond is formed.
ionic |
kinetic |
covalent |
hydrogen |
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Question 421 pts
Changing the number of ____________ of an atom would change the chemical properties of the atom.
neutrons |
protons |
electrons |
electron shells |
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Question 431 pts
Forming molecules and breaking down molecules in biological organisms usually requires the use of _________ to help the reaction proceed faster.
oil |
heat |
enzymes |
blood |
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Question 441 pts
Organic molecules have a core composed of .
carbon |
nitrogen |
phosphorus |
hydrogen |
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Question 451 pts
The building blocks of carbohydrates are
polypeptides |
amino acids |
nucleotides |
monosaccharides |
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Question 461 pts
Water molecules crossing a membrane from high to low concentration is .
active transport |
cell fate |
facilitated diffusion |
osmosis |
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Question 471 pts
Choose the membrane molecule responsible for the passage of polar molecules and ions into and out of the cell.
phospholipids |
transmembrane channel proteins |
cell surface proteins |
carbohydrate chains |
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Question 481 pts
The simplest cells are .
prokaryotic |
animal cells |
eukaryotic |
cells of fungi |
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Question 491 pts
Which of the following membrane bound organelles are found inside bacterial cells:
nucleus |
organelles are not found in prokaryotic cells |
mitochondria |
chloroplasts |
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Question 501 pts
Which of the following is a specialized components of the cell, is associated with the rough endoplasmic reticulum, and is responsible for making proteins.
vacuoles |
ribosomes |
golgi complex |
nucleus |
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Question 511 pts
Proteins are sorted, modified, and packaged by the _____________ and later transported to the outside of the cell.
ribosomes |
golgi bodies (golgi apparatus) |
nucleus |
mitochondria |
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Question 521 pts
Dehydration synthesis is a process of linking two smaller subunits together to form a polymer. Which of the following statements below is true of dehydration synthesis?
Oxygen is consumed. |
A water molecule is removed from the molecules. |
A water molecule is added to the molecules |
Carbon dioxide is given off. |
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Question 531 pts
Proteins are made up of _________ held together by peptide bonds.
monomers |
monosaccharides |
polymers |
amino acids |
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Question 541 pts
Which of the following gives an amino acid its properties?
NH2 |
R Group or functional group |
COOH |
H |
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Question 551 pts
The final three-dimensional shape of a protein that includes the bonding of two or more polypeptide chains is call its _____ structure.
Tertiary |
Quaternary |
Secondary |
Primary |
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Question 561 pts
How do DNA and RNA differ?
all statements are differences between DNA and RNA. |
DNA is double stranded while RNA is single stranded. |
They have different sugars. |
Thymine is present in DNA but not in RNA. |
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Question 571 pts
Which of the following is a component of a DNA nucleotide?
phosphate |
a nitrogen containing base |
All are components of a nucleotide. |
5-carbon sugar |
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Question 581 pts
Cell membranes are made up of several different types of molecules. Select the membrane molecule below that is made up of a polar region and two non-polar fatty acid tails.
transmembrane proteins |
carbohydrate chains |
phospholipids |
cell surface proteins |
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Question 591 pts
Two organelles which are believed to have once been free-living bacterial cells are ____________ and ______________.
chloroplasts and mitochondria |
golgi apparatus and endoplasmic reticulum |
ribosomes and nucleolus |
peroxisomes and lysosomes |
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Question 601 pts
The is an extensive system of internal membranes responsible for producing carbohydrates and lipids.
smooth endoplasmic reticulum |
rough endoplasmic reticulum |
nucleolus |
mitochondria |