BIOL2000 Lecture Notes - Lecture 13: Cistron, Membrane Transport Protein, Periplasm

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yellowhippopotamus301

16 Jan 2017

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Gene expression can be regulated at any stage from mrna synthesis to protein activity. Transcriptional control: common bc energy saving but slow. Translation: least common in bacteria but control levels of ribosomal proteins. Post trans regulation: need speedy control, energy expensive but fast. Activate or inhibit protein by chem modify. Control points of gene activity for protein encoding genes. Degrade rna after transcription: low rna level. Rate of transcription/degradation: controls amount of rna. Modify active protein: degrade or re localize. Rate of degredation: controlled by post trans events like polyubiq. Other modifications: add or remove phosphates like cdk. Rna abundance: northern or rtpcr: nucliec acid proce or detect rna. Transcript: akter, translatin: no change, post trans: no change. Protein abundance: western: measure abundnace of protein. Transcript: alter, translation: alter, post trands: depends on effect. Localization, mobility in western, interact w binding partners. ), translate: no change (absent>), post trans: alter. If after translation: wouldnt see change in rna.

Related Questions

QUESTION 10

If you can drink milk as an adult, it means that you have inherited a mutation in the promoter of your lactase gene (the gene that encodes the enzyme you need to break down lactose). Predict the effect of this mutation:

		The mutation changes the number of domains in the enzyme, which makes it work more efficiently
		The mutation changes the amino acid sequence of the lactase protein
		The mutation increases the number of copies of the lactase gene that will be found in your genome
		The mutation changes whether the lactase sequence is found in an intron or exon
		The mutation affects the expression of the lactase gene

1.2 points

QUESTION 11

A competitive inhibitor is decreasing the activity of an enzyme. Predict the effect of adding more substrate to the reaction.

		The substrate will increase the reaction rate by binding to the allosteric site
		The substrate will increase the reaction rate by competing with the inhibitor for the active site
		The reaction rate will not change unless the inhibitor can be removed
		The enzyme adjusts its shape so that the substrate, but NOT the competitive inhibitor, can bind
		The substrate will bind to the competitive inhibitor and block its ability to bind to the enzyme

1.2 points

QUESTION 12

What determines where in the genome a transcription regulator will bind?

		Transcription regulators bind to the 5' UTR region of a gene
		Regulators bind via complementary base-pairing to certain DNA molecules
		Covalent bonds form between the transcription regulator and the atoms of the DNA backbone
		Every eukaryotic gene has a different transcription regulator that will bind to the 5' end of the gene
		Transcription regulators bind to specific DNA sequences via multiple weak non-covalent interactions

1.2 points

QUESTION 13

What is the basic premise of cell theory?

		DNA -> RNA -> protein
		All cells arise from pre-existing cells
		DNA provides the complete instructions to create a cell
		The identity of a cell is determined through gene expression patterns
		All cells contain the same four basic macromolecules

1.2 points

QUESTION 14

What is the benefit of using BOTH the lac activator and the lac repressor to control gene expression?

		Using both an activator and repressor enables cells to more accurately determine the amount of lactose available in the environment
		Enzymes to digest lactose are only made when energy is low and lactose is available
		The activator can override the inhibition of the lac operon by the repressor
		The repressor can control the enhancer, while the activator can control the promoter
		When neither the lac activator or repressor is present, expression of the lac operon is too high

1.2 points

QUESTION 15

What is the histone code used for?

		Phosphorylation and acetylation of DNA affect its ability to be compacted
		Changes to the sequence of DNA change whether DNA will wrap around histone proteins
		Covalent modifications of histones affect the ability of the transcription initiation complex to form
		Histones provide the codon sequences needed for translation to occur
		The histone code affects which amino acids will get added to a polypeptide

BIOL2000 Lecture Notes - Lecture 13: Cistron, Membrane Transport Protein, Periplasm

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