BIOL2000 Lecture Notes - Lecture 17: Chromatin Remodeling, Cpg Site, Deoxyribonuclease I
16 Jan 2017
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Document Summary
Variety of mechanism control gene expression in eukaryotes. Transcriptional regualtion and post trans control are most common. Liek prok, euk can control gene expression at each level. Bacteria: not nucleosome but super coiled dna in bacteria. Expression is regulated by state of local coiling. Opeong: group of genes transcribed as single unit. If super coiling in that region: regulated. Cant turn on until mutation changes super coiling around it. Packaging of dna in bacteria can affect transcription. Rna is more stable if it takes longer to destroy. Bacteria: introns are rare, some self splicing. Ribozyme: rna molecule with catalytic activity: splices itself. E coli and bacteriopahge stabilty changes dueing infection process on northern blots after transcript inhibition. Dont waste time making bacterial proteins bc bacteria will die. Chromatin structure: impediment to transcription in eukaryotes. Bacteria can regulate mrna half life when bacteria phage infects bactera. Linker dna, wrap around protein attached to linker dna and nuclesome.
