Biology 1002B Lecture Notes - Lecture 19: Genbank, National Center For Biotechnology Information, Wild Type
31 May 2016
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Increase temperature to boiling denatures and splits dna into 2 strands. When temperature decreases short dna sequences known as primers bind or anneal to sequence: target to be copied. At higher temp taq polymerase blinds to primer and extends. Cycle is repeated to make additional copies. After 3 cycles target sequences required are produced. Where to get channelrhodopsin sequence: look for mutants (in this case in regards to phototaxis, discover the site of the mutation. Did this to find channelrhodopsin (codes for opsin) The problem of redundancy (problem of finding gene from protein: metseralatrpglyval gene sequence. Sequence is a continuous, the eyfp is attached to the channelrhodopsin (absorbs different wave of light) A lot of times if you stick a tag on a protein, the original protein wont work: works this time. Ncbi: repository of molecular information (dna, proteins, genomes, deposit and search databases (genbank ) Pretty much has all sequences that we know of.
