Biology 1002B Lecture Notes - Lecture 19: Taq Polymerase, Polymerase Chain Reaction, Channelrhodopsin

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Genetics start at genes and figure out the protein. Mutants of chlamydomonas were defective and did not respond to light. Biochemistry (protein based) start at the eyespot and grind up lots of chlamydomonas and isolate lots of proteins of the eyespot and study them. It is very hard because of the redundancy in the genetic code. Many codons code for one amino acid: 1 codon (aug) codes for methionine. But: 6 possible codons code for serine, 4 possible codons to code for alanine. 43=64 possible combinations specifying 20 aa (__ __ __) 42=16 < 20 you need 43 combinations (__ __) Some codons used more preferentially than others. How"d you get a sequence without any introns equivalent to a. Dna sequence equivalent to mrna (codons you need to make the functional protein). Not the genomic sequence (no introns & no promoter) Only one stop codon because eyfp gene is fused in frame with.

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