BIO230H1 Lecture Notes - Lecture 9: Hsp70, Peroxisomal Targeting Signal, Thylakoid

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19 Oct 2016
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BIO230H1 Full Course Notes
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Extracellular matrix: specialized material outside cell wall. Proteins must be sorted to the correct location as protein synthesis is initiated on ribosomes in cytosol. Movement of proteins within cell from different compartments. Into cell: proteins fully synthesized in cytosol before sorting, unfolded: mitochondria, plastids, folded: nucleus, peroxisomes, co-translational process, endoplasmic reticulum (er, proteins with er signal sequence are associated with er during protein synthesis. Gated transport: can go both ways. Transmembrane transport: requires protein translocators, one way. Vesicular transport: vesicles move protein between compartments, some both ways, some one way. Nuclear pore complex: selective transport of macromolecules (rna, proteins, free diffusion of small molecules (less than 5000 daltons) Transport of cargo (things that move, i. e. , proteins) through npc in both directions: nuclear import: from cytosol to nucleus, nuclear export, from nucleus to cytosol. Regulation of ran gtpase in the nucleus and cytosol. Ran gtpase cycles between gdp-bound and gtp bound.

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