BIO315H5 Lecture Notes - Lecture 24: Rhoa, Nuclear Membrane, Nuclear Lamina
Document Summary
Once sister chromatids are attached to the spindle, spindle can move the chromatids. Kinetochore proteins induce depolymerization at the plus end - plus end is not capped, its free so its free to depolymerize or polymerize - generates a force that pulls the kinetochore towards the spindle core. Microtubule flux - dots represent newly incorporated tubulin subunits that move away because with microtubule flux you have microtubule changing - minus end may be depolymerizing while plus end at sister chromatids is polymerizing. Over time, it moves towards the poles - due to depolymerization at the minus end. Generates force that pulls kinetochore towards the poles. Polar ejection force/polar wind - there are chromokinesins (4 and 10) - plus end directed motors. Cargo is arms of sister chromatids and move towards plus end of spindle microtubules - towards centre of the spindle. Important during metaphase and prometaphase - to align at metaphase plate.
