BIO315H5 Lecture Notes - Lecture 17: Peptide, Lamellipodium, Focal Adhesion

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20 Nov 2017
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By its dynamic properties and its dynamic polarity. By controlling where and how actin assembles, the cell harnesses polar and dynamic properties of filaments to perform functions. Explain how figure 16. 13: the time course of actin polymers in a test tube, explain the nucleation, polymerization and depolymerization of actin filaments. This figure shows that regulation of actin filament formation is an important mechanism for function. Polymerization of pure actin subunits into filaments occurs after a lag phase. Polymerization occurs more rapidly in the presence of preformed fragments of actin filaments, which act as nuclei for filament growth. The % free subunits after polymerization reflects the critical concentration cc, at which there is no net change in polymer. Actin polymerization is often studied by observing the change in the light emission from a fluorescent probe, called pyrene, that has been covalently attached to the actin.

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