BIO206H5 Lecture Notes - Lecture 6: Okazaki Fragments, Dna Ligase, Semiconservative Replication
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Bio206 tutorial replication: when discussing dna replication, explain what is meant by the terms, conservative : daughter cells have newly synth. When he allowed dna replication to proceed for a longer period of time, he found a lo(cid:449)e(cid:396) f(cid:396)e(cid:395)ue(cid:374)(cid:272)(cid:455) of these sho(cid:396)t f(cid:396)ag(cid:373)e(cid:374)ts a(cid:374)d lo(cid:374)g si(cid:374)gle st(cid:396)a(cid:374)ded dna (cid:272)hai(cid:374)s afte(cid:396) e(cid:454)t(cid:396)a(cid:272)tio(cid:374) a(cid:374)d denaturation. Explain how this result might be related to the fact that all known dna polymerases synthesize. Dna is replicated in short segments that are glued together by dna ligase. Dna replication is bidirectional i(cid:374) (cid:449)hi(cid:272)h the(cid:396)e a(cid:396)e (cid:373)a(cid:374)(cid:455) poi(cid:374)ts that it (cid:272)a(cid:374) o(cid:272)(cid:272)u(cid:396). Elongation and cofactors required for polymerase iii, dnaz protein, Rnapii, ca150 elongation and dna elongation factors i and iii. Amount of energy consumed per nucleotide higher addition datp lower: eukaryotes have a significant amount of dna packaging. This makes the sequence more complex, making the proteins able to bind with the dna.