BIO206H5 Lecture Notes - Lecture 5: Mature Messenger Rna, Methyltransferase, Caat Box
Document Summary
Ecor1 a protein that is capable of binding dna, not randomly but at a specific nucleotide sequence and it is a protein catalyst reaction that the protein catalyzes is phosphodiester bonds. It is a homodimer made up of two identical subunits; catalytic activity requires cofactor which is magnesium. Presence of magnesium will cleave the phosphodiester bond in two places, breaking. Enzymes that cleave phosphodiester bonds between nucleotides of nucleic acids in dna or rna. Exo(cid:374)u(cid:272)leases: (cid:272)ut (cid:374)u(cid:272)lei(cid:272) a(cid:272)ids f(cid:396)o(cid:373) the 5(cid:859) o(cid:396) (cid:858)3 e(cid:374)d of the (cid:373)ole(cid:272)ule degrades (binds at the ends and chew towards the middle) Endonucleases: cut nucleic acids internally degrades (bind to a region and they cut it inside) Subgroup named restriction enzymes (restriction endonucleases) famous for two reasons: Native to bacteria (bacterial defense against viral invasion) bacteria also have. A subset of endonuclease enzymes that cut dsdna at specific dna sequences identified by the enzyme itself.
