BIO 3124 Lecture Notes - Lecture 8: Vital Stain, Hemocytometer, Absorbance
10 Dec 2016
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How to generate the data for the y-axis. Eithe(cid:396) tells (cid:455)ou ho(cid:449) (cid:373)u(cid:272)h light (cid:449)e(cid:374)t th(cid:396)ough o(cid:396) ho(cid:449) (cid:373)u(cid:272)h light did(cid:374)"t go th(cid:396)ough. The higher the density of the cells, the higher your optical density, or the lower the transmission. Things can scatter light (eg. mud in tube) If want to compare microbial load in 2 diffe(cid:396)e(cid:374)t lakes it"s (cid:374)ot (cid:448)alid (cid:271)e(cid:272)ause (cid:455)ou do(cid:374)"t k(cid:374)o(cid:449) (cid:449)hat else is in the lake that could interfere with light. This would be used in a lab, same medium and bacteria and from same place, you just want to know which has more. Counting chamber has 3 dimensions (l, w, d), 3 dimensions allow it to contain a max. volume. Do(cid:374)"t consider it how much is applied, consider what v the counting chamber represents. Once you have the # of cells you observe and have dimensions you can determine # of cells.
