BIO 1140 Lecture Notes - Lecture 17: Signal Recognition Particle, Signal Peptidase, G Protein–Coupled Receptor

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We talked about how mrna would assemble with ribosomes and iniiate translaion but we didn"t talk about where this happens inside the cell and what are the diferent possibiliies. So we"re going to look at the two diferent possibiliies this morning. So our free ribosomes in the cytosol, the small ribosomal subunit will interact with the methionine trna, the mrna will be assembled from the g-cap, bringing in the large ribosomal subunit and translaion will occur. Those proteins will end up being soluble proteins, globular, free cytosolic proteins. They"re going to stay inside the cell, mainly in the cytoplasm. There are some ways to import them in various organelles, paricularly the mitochondria but we"re not going to cover those aspects in this course. Now what if you need to prepare a protein that has to be an integral protein, a peripheral protein, a lipid-anchored protein, a transmembrane protein.

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