MBG 2040 Lecture Notes - Lecture 1: Octal, Tata-Binding Protein, Spliceosome
2 Nov 2016
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Document Summary
The central dogma of molecular biology as first articulated by francis crick in 1958. The discovery of reverse transcriptase in retroviruses and retro-like viruses (howard temin and david. The standard b-form dna helix of watson and crick. There are five types of dna polymerases in e. coli: polymerases i, ii, iii, iv and v, polymerase i and iii aid in chromosomal dna replication, ii, iv, v are dna repair functions. Pol i: removes the rna primer: has 5"-3" polymerase and exonuclease activity, can proofread strands due to its 3"-5" exonuclease activity, not highly processive meaning it can only synthesize short fragments of dna. Pol iii: the main replicative polymerase, highly processive: has 5"-3" polymerase activity but no 5"-3" exonuclease activity, proof reading has 3"-5" exonuclease activity. Fidelity of dna replication: one mistake per 104 - 105 nucleotides added; with proof-reading, the final error rate is 108 109. Dna polymerase i: 5" 3" polymerase activity.
