BIOL207 Lecture 30: b207lect30_1 copy.pdf

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limehippopotamus462

4 Dec 2014

School

University of Alberta

Department

Biology (Biological Sciences)

Course

BIOL207

Professor

Cameron Harrington

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Document Summary

Lecture 30 - fluorescence in situ hybridization: how to fish, fish with centromere probes, fish with gene probes, fish with chromosome paint probes, multicolour karyotyping, how to fish, overview. Identify genes or chromosomes amongst interphase or metaphase chromosomes: procedure. Step 1 obtain one (or more) bac clones. Step 2 make a probe with a random prime labeling reaction: one (or more) bac clones one (or more) fluorescently labeled nucleotides. Step 3 put some cells onto a slide, denature the dna, add the probe: results. A b c: fish with centromere probes. Example 1: cell = amniotic (fetal) cell blue (dapi) = dna green = chr 13 cen red = chr 21 cen. Incidence of trisomy per 100,000 pregnancies spontaneous chr abortions. Table 18-2, griffiths et al. , 2000 live births. Incidence of sex chromosome aneuploidy per 100,000 pregnancies genotype. Table 18-2, griffiths et al. , 2000 spontaneous abortions.

Related Questions

trouble with genetics problem that has several genes, I cant seem to set it up right.

In clown fish there is a gene that controls their preference for red or yellow sea anemones. Interestingly, the preference for red sea anemones is dominant in males but recessive in females. a third allele exists that gives the fish a preference green anemones that is recessive to all other known alleles in either sex.

The gene that controls the color of the clown fish is found on the X chromosome. There is an allele that causes the fish to have a lemon color, which is dominant to a ruby color. Both lemon and ruby are dominant to the white color allele. You notice that regardless for the genetics of preference; if the female fish is lemon she will always prefer the yellow sea anemone. Assume fish follow the sex determination as mammals.

a) What would be the phenotypic outsome of a:

Male cherry fish who prefers red anemones whose dad was homozygous ruby and has preference for green anemones.

Female lemon fish that prefers yellow anemones who has the same father as the male fish.

b) Explain how the genetic illustrated above have a fitness advantage for these fish.

emeraldweasel173

You wanted to know whether the expression of a specific gene in different areas of the brain could enhance memory. You are working with fruit flies and have devised a simple memory test that can be conducted with ease. Describe how you would achieve specific expression using the GAL 4 UAS system and why this would be a powerful approach. In addition describe how you would use in situ hybridization to confirm that you were expressing the gene where you thought you were. Finally describe how the second part would be done differently if you used GFP as a reporter gene instead on in situ hybridization.

limeskunk697

Hello could you help me with the following questions.

1. Which strategy for testing gene function requires that you know more about the gene or pathway you are studying before you start?

a. Forward genetics

b. Reverse genetics

2. Can the Cre-loxP system be used to "knock out" a gene in a wildtype (not genetically modified) mouse?

a. Yes, the Cre recombinase can use any loxP sites, even those that have not been engineered into the genome.

b. No, the Cre-loxP system requires loxP sites to be inserted into the genome by researchers.

3. What is the mechanistic reason that in situ hybridization images are purple?

a. Binding of RNA to a probe results in a purple precipitate.

b. An antibody conjugated to alkaline phosphatase cleaves NBT/BCIP into a purple product.

c. NBT/BCIP in solution is purple.

d. Digoxigenin-labeled nucleotides in the probe react with NBT/BCIP to form a purple product.

4. In situ hybridization detects:

a. RNA

b. Both RNA and protein

c. protein

d. DNA

5. Which is NOT a mechanism for control of mRNA expression by cytoplasmic localization?

a. Localized protection

b. Nuclear export and splicing

c. Active transport along the cytoskeleton

d. Diffusion and local trapping or anchoring

BIOL207 Lecture 30: b207lect30_1 copy.pdf

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