BIOCH200 Lecture Notes - Lecture 26: Oxidative Phosphorylation, Atp Synthase, Mitochondrial Matrix
Document Summary
Chemical energy in the phosphoanhydride bonds of atp. Oxidative phosphorylation: the action of atp synthase: 10 protons are generated from nadh. The o is the first letter of oligomyocin, which is sensitive to fo. One turn of f1 makes 3 atp. Rate of atp determines the proton movement, and hence, oxygen consumption. Does not depend on gradient (gradient acts like the gas for the car and basically makes the reaction go faster) 3 active subunits involved, 6 subunits overall: the 3 subunits of atp synthase. Every complete turn of the central shaft is associated with the generation of 3. As the fo rotates, the conformation changes to generate atp. The adenine nucleotide translocase and the pi-h+ symport: import of 1 h+/atp synthesized = 4 h+ overall per atp. Newly synthesized atp is explored form the mitochondrial matrix into the cytosol. As atp is exported, adp + pi is imported. Gradient helps to export atp better than importing adp and pi.