BIOCH200 Lecture Notes - Lecture 32: Oxidative Decarboxylation, Alpha-Ketoglutaric Acid, Pyruvic Acid

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Biochem 200 - lecture #32 - regulation of citric acid cycle. Regulation of the citric acid cycle: no rate-limiting reactions. Affected by concentrations of intermediates: regulated enzymes. Both enzymes are involved in oxidative decarboxylation: inhibitors. Nad+/nadh ratio affects oxidation reactions as well: substrate-product effects, decreased amount of adp in the matrix. Atp synthase is only going to let molecules back into the matrix if making atp. If (cid:1373) [adp], protons are not going to enter the gradient and there will be no. Even if atp/adp does not affect this rate, protons will affect it anyways reduction of oxygen: increased amount of adp in the matrix. Atp synthase lets the protons go through the imm into the matrix. Anabolic = amphibolic: cac intermediates can be used in the synthesis of amino acids, carbs, fats, nucleotides and other compounds, need oxaloacetate to continue making energy. Must be adequate intermediates: many reactions may be anapleutoric. Pyruvate (cid:1372) oaa (immediate source of energy)

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