JOUR 3440 Lecture Notes - Lecture 3: Heterochromatin, Chloroplast, Chymosin

41 views20 pages

coffeeoyster95

24 Oct 2015

School

Department

Course

Professor

For unlimited access to Class Notes, a Class+ subscription is required.

Document Summary

Polymerase chain reaction: molecular biological technique for amplifying (creating multiple copies of) dna without using a living organism. Activators: bind to promoter dna elements (cis) and activate transcription. Repressors: complex- multiple ways to turn genes off. 3a the method used in the generation of transgenic organisms. Often the introduced gene is put into the genome of an organism. 4a the term often used when the recipient is eukaryotic cell. Gene may be inactivated due to dna methylation. Gene may lack promoter / enhancer sequences. Integration occurs close to heterochromatin ("silencers") or insulators. 7a an enzyme that occurs in gastric juice; causes milk to coagulate; Adv: usually only one insert (copy number), large size of. Dna can be inserted, using nature- very efficient. Disadv: works well on dicots, more difficult with monocots (need embryos). Can get gene into chloroplasts (many adv of chloroplast), works on all plants.

Related Questions

Polymerase chain reaction is known for its power of amplifying atarget DNA sequence at a high speed. Each cycle can double thenumber of DNA molecules (target sequence). Which of the followingis CORRECT regarding PCR?

A. In order to make 10 copies of the DNA, you need at least 4cycles of PCR.
B. Helicase is required to separate the two strands in PCR.
C. Dideoxynucleotides are used in PCR.
D. RNA primers are needed in PCR.
E. All of the above.

greypanda589

The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park, in Wyoming. This organism contains a heat-stable form of DNA polymerase known as Taq polymerase, which continues to function even after it has been heated to 95Â°C. Why would such a heat-stable polymerase be beneficial in PCR?

What would happen if it werenât heat stable?

How might you choose a region of DNA for a PCR primer so as to increase the temperature necessary for primer annealing (to minimize nonspecific PCR products)?

A PCR reaction begins with 5 double stranded segment of DNA. Estimate the number of double-stranded copies of DNA that are present after the completion of 15 amplification cycles?

1.The PCR (polymerase chain reaction) protocol that is currentlyused in laboratories was facilitated by the discovery of abacterium called Thermus aquaticus in a hot spring insideYellowstone National Park, in Wyoming. This organism contains aheat-stable form of DNA polymerase known as Taqpolymerase, which continues to function even after it has beenheated to 95Â°C.

a.Why would such a heat-stable polymerase be beneficial inPCR?

b.What would happen if it werenâtheat stable?

c.How might you choose a regionof DNA for a PCR primer so as to increase the temperature necessaryfor primer annealing (to minimize nonspecific PCRproducts)?

d.A PCR reaction begins with 5double stranded segment of DNA. Estimate the number ofdouble-stranded copies of DNA that are present after the completionof 15 amplification cycles?

plumwhale64

JOUR 3440 Lecture Notes - Lecture 3: Heterochromatin, Chloroplast, Chymosin

Document Summary

Get access

Related Documents

JOUR 3440 Study Guide - Final Guide: Dicotyledon, Generally Recognized As Safe, Gene Gun

JOUR 3440 Study Guide - Final Guide: Microtubule, P53, Growth Factor

LSCI 204 Lecture Notes - Lecture 22: Orites, Lac Operon, Cloning

Related Questions