BIO 214 Study Guide - Quiz Guide: Proofreading, Mutagen, Dna Supercoil
Document Summary
Overview on transposable elements and plasmid dna and vector (including images from the textbook for better explanation) Some dna sequences move from one position to another. Make up more than 40% of human genome. Typically an insertion mutation of a fragment into a gene. Transposase (enzyme) that can self insert dna into the genome. Both sides contain insertion sequences inverted repeats. Able to insert itself into the host genome. There can be different types of transposons such as tn5, tn10, tn7, etc . Recognition sequences on the host cell through a direct repeat. Inverted repeats: same repeats but opposite of each other. Develop in absence of any added agent. Rise randomly during dna replication, damage to dna or insertion of transposons. Develop after exposure to a mutagen to dna or interfere with dna repair mechanisms. Correction of errors in base pairing made during replication. Other repair mechanisms repair incorrect pairings and dna damage.
