1. (20pts) Letรขยยs consider how restrictive enzymes work. Look the two samples of DNA shown below:
DNA Sample #1
5รขยยรขยย T C C A G T G A T C T C G A A T T C G C T A G T A A C G T T C G A T
3รขยยรขยย A G G T C A C T A G A G C T T A A G C G A T C A T T G C A A G C T A
DNA Sample #2
5รขยยรขยย T C A T C G A A T T C C T G G A A T C A G C A G A A T T C G C A T A
3รขยยรขยย A G T A G C T T A A G G A C C T T A G T C G T C T T A A G C G T A T
If both samples are treated with the restriction enzyme EcoRI [recognition sequence GรขยยAATTC] then indicate the number of fragments and the size of each fragment from each sample of DNA. List the fragment sizes in order of largest to smallest.
Sample # 1 Sample # 2
# of fragments:________ # of fragments:________
Sizes of fragments (largeร small): Sizes of fragments (largeร small):
(20pts) Read the protocol for Lab 8B.
What is supercoiled DNA
Why would it migrate further into an agarose gel if itรขยยs uncut plasmid DNA?
What causes this type of DNA to form?
Is this likely to occur to our isolated plasmid DNA?