CH ENGR C125 Study Guide - Quiz Guide: Theoretical Plate, Capacity Factor

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9 Jan 2019

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Compound x: the following data applies to a column for liquid chromatography. Length of column is 35. 7 cm, the volume of the mobile and stationary phase is 1. 6 ml and 0. 134 ml, respectively. The column was run at a flow rate of 0. 5 ml/min. A mixture of 4 components (a, b, c, d) with the following chromatography data: 20. 6: component b and c are the closest component that may affect the separation. Determine the extent to which each molecule interacts with the stationary phase, the capacity factor (k") of b and c: determine the resolution (r) and the relative retention ( ) between components b and c. Was good separation achieved: text book problem no.

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A gas chromatography column was operated under the following conditions:

Column dimension: 1.1 M length x 2.0 mm internal diameter Retention times: Nonretained carrier gas, tm = 0.30 min Compound A retention time, trA = 1.98 min Compound B retetntion time trb = 4.16 min Compound C retention time, trc = 7.93 min Peak widths at base : 0.18, 0.41, 0.82 min for A, B, and C respectively.

Calculate: i) the retention factor, kâ for each compound ii) the selectivity factor, Î± for each adjacent pair of compounds iii)

1. A four component mixture is separated using a liquid chromatography column 25.0 cm long, at a flow rate of 0.500 mL/min, and a dead time of 3.10 minutes. For this particular column the volume of mobile phase in the column is 1.550 mL, and the volume of the stationary phase in the column is 0.164 mL. The peaks obtained in the chromatogram are separated as follows: (A) elutes in 5.47 minutes with a peak width (defined as 4Ï) of 0.41 minutes (B) elutes in 13.11 minutes with a peak width of 1.06 minutes (C) elutes in 14.25 minutes with a peak width of 1.08 minutes (D) elutes in 21.62 minutes with a peak width of 1.72 minutes.

Calculate the chromatographic resolution of peaks B and C and report this value to three significant figures using scientific notation (e.g. 0.0012 is reported 1.2E-3).

2. A four component mixture is separated using a liquid chromatography column 25.0 cm long, at a flow rate of 0.5 mL/min. For this particular column the volume of mobile phase in the column is 1.550 mL, and the volume of the stationary phase in the column is 0.164 mL. The peaks obtained in the chromatogram are separated as follows: (A) elutes in 5.47 minutes with a peak width of 0.41 minutes (B) elutes in 12.79 minutes with a peak width of 1.07 minutes (C) elutes in 14.11 minutes with a peak width of 1.16 minutes (D) elutes in 21.62 minutes with a peak width of 1.72 minutes. Unretained compounds elute from the column in 3.64 minutes.

Calculate the retention factor of peak B and report this value to three significant figures using scientific notation (e.g. 1,230 is reported 1.23E3).

For a gas chromatography separation of two components, A and B, the following data were collected. An un-retained peak had a transport time of 5 seconds through the column.

	A	B
Retention time (s)	25	30
Peak Width at Half-Height (FWHM, s)	2.5	3.0

(a) What are the capacity factors (k') for A and B?

(b) What are the number of theoretical plates (N) and plate height (H) for A and B?

(d) What is the resolution of these two peaks?

(e) Based on your answers to (c) and (d), are these peaks sufficiently separated? Explain.

CH ENGR C125 Study Guide - Quiz Guide: Theoretical Plate, Capacity Factor

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