CHEM 212 Lecture Notes - Lecture 29: Size-Exclusion Chromatography, Gas Chromatography, High-Performance Liquid Chromatography

• School: University of Victoria
• Department: Chemistry
• Course: CHEM 212
• Professor: Hamilton M.Coreen

Document Summary

Chromatography a bunch of liquid liquid separations seperation technique two immiscible phases more versatility --> two phases stationary mobile. Liquid chromatography --> liquid moving phase sample infused into mobile phase. Instrumental technique column is on its side mobile phase out collection or detection size of peak from instrument gives us quantitative data also gives us qualitative --> from when it appears out of column. Once particles get too small though, the solvent won"t go through anymore --> apply pressure hplc. Sample components do not adsorb onto surface but actually dissolve. Like dissolves like components come out last are the polar compounds non-polar stationary phase --> components out last are non-polar. 3: a nity chromatography, not a mainstream technique yet, stationary phase has deisgner molecules, lots of biochemical application. Make stationary phase customized for a certain molecule. Get good separation of the special molecule. Everything we need is printed o from chromatogram.