BIO 203 Study Guide - Midterm Guide: Cytochrome B6F Complex, Citric Acid Cycle, Pyruvate Dehydrogenase

Explain why each of the following is advantageous to the cell (1) allosteric inhibition of phosphofructose kinase by citrate (2) allosteric activation of isocitrate by ADP. Provide a possible allosteric inhibitor and allosteric activator of pyruvate dehydrogenase and explain why each would be advantageous. (6)

Please be straight-forward and to the point, but also enough detail that it actually answers the question and how you got to that answer. Thanks so much, I'll be sure to rate the answers! :)

Suppose NAD+ was unavailable due to a mutation in the NADH dehydrogenase. If FAD could substitute, how would this affect ATP production? (Give specific numbers.) Based on the redox potentials, would this be possible?

1. Choose the TWO correct statements describing the citrate cycle.

2.Which molecule in the net reaction of the citrate cycle contributes to the inhibition of pyruvate dehydrogenase?

3. How would a high NADH to NAD⁺ ratio be expected to affect the pyruvate dehydrogenase reaction?