BIO ENG 111 Study Guide - Midterm Guide: Scanning Probe Microscopy, Tandem Mass Spectrometry, Sanger Sequencing
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Question 0: fill in your name and student id on each page. (1) Question 1: briefly define the following terms (15): tandem mass spec (3 points) First a sample is ionized and separated by mass- to-charge ratio (m/z). Ions of a particular m/z generated in the first step are then fragmented in the second step, resulting in a new spectrum of ions. The characteristic fragmentation pattern from the parent ion provides structural information about a peptide. Tandem mass spectrometry can be used to sequence and identify unknown peptides and for proteomic analysis. Atomic force microscopy is a type of scanning probe microscopy that uses a cantilever with an extremely fine tip to characterize surfaces with up to near atomic resolution. Afm can be used for both topographical imaging and mechanical characterizations such as stiffness or force measurements. +1 sharply tipped cantilever interacts with surface of material. +1 application: shotgun dna sequencing (3 points)
