BIO 2306 Study Guide - Quiz Guide: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Ti Plasmid
Document Summary
Techniques that locate/isolate/alter/study dna: basic molecular techniques, restriction enzymes [restriction endonucleases] Double-stranded cuts @ specific seq. called palindromic seq. Extra info: complementary sticky ends that pair if mixed together fragments can be sealed by dna ligase. Nicks in the sugar-phosphate backbone of the 2. If dna molecules cut by same r. e. Separates dna fragments by size: dna samples containing different sized fragments, all dna fragments move toward positive pole, dye added to the gel; dna fragments appear as bands. Place in wells (in an agarose gel) Transfers dna fragments from gel to solid medium. Then, a dna sequence can be located by probing w/a: dna fragments separated by gel electrophoresis, gel soaked in alkali solution. Kanye west eats protein : gene cloning = produce identical copies (clones) of a gene. Gene placed into host genome (bacteria) to be replicated cloning vector : carries foreign gene into host cell.
