Biology 2382B Study Guide - Final Guide: Dynactin, Axoneme, Atp Hydrolysis
19 Aug 2017
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Document Summary
Microtubules: tracks for transport: vesicle transport (bi-directional, motor proteins require energy (atp) Squid axons can measure how fast things move. Labelled proteins travel at different speeds in cells (not diffusion: messages made in the cell body goes towards the + end of the microtubule down the axon. Kinesin, mt"s plus (+) end directed motor protein: many types (14 known classes, 45 genes in humans) 2 heavy chains: head, flexible neck (linker) and stalk. 2 light chains (variable: heavy chain heads have atpase activity and mt binding ability. Light chains recognize cargo: most (+) directed. Kinesin moves things to + end of microtubules. 4 proteins represents some banding patterns on the gel: heavy chains recognize microtubules. 2 heavy chains come together head is an atpase, binds to microtubules, uses atp to move along the microtubule. Light chains recognizes cargo: different light chains recognize different cargos. Structures and functions of selected kinesin members: head domains are always atpases.
