BIO130H1 Study Guide - Final Guide: Atp-Binding Cassette Transporter, Active Transport, X-Ray Crystallography
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BIO130H1 Full Course Notes
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Document Summary
Lecture 10: cellular form and function the parts of a cell. Not in animal cells: chloroplasts, vacuoles, cell wall. The bilayer contains two leaflets/faces/layers of amphiphilic phospholipids, which are synthesized in the er. The two faces are called the exoplasmic face and cytosolic face. The lipid bilayer is a fluid structure. Fluidity increases with: temperature: more unsaturated bonds, shorter tails. Cholesterol (steroids) decreases the mobility and permeability of the pm. Glycolipids, synthesized in the golgi, are found in the outer leaflet and in some organelles. Phospholipids rapidly diffuse laterally, but only flip-flops with the help of a translocator protein. Fluorescence recover after photobleaching (frap) is used to study protein movement. Cell membrane proteins: transmembrane, integral, and peripheral proteins: single -helix (receptors, multiple -helices (ion channels, (cid:533)-barrel (rigid channels, anchored protein by amphiphilic -helix, lipid anchored (cytosolic face, gpi lipid anchored (cell surface, peripheral (non-covalent interactions) Protein identification by x-ray crystallography and hydrophobicity plots.
