BIO120H1 Study Guide - Quiz Guide: Glucose Oxidase, Blood Sugar, Spectrophotometry
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BIO120H1 Full Course Notes
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Lab 1 assaying biomolecules: measuring glucose in serum for ogtt. Require the detection of a signal that correlates with the amount of the biomolecule in the. Signal should be specific for the biomolecule in question and relatively easy to detect using lab. 2 different types of signals possible: measured by using an inherent property of the test substance ex: the absorption of red light by chlorophyll a can be measured and used to quantitate its concentration in solution. A spectrophotometer can be used to measure absorbance at 540 nm and a standard curve can be generated to set the relationship between absorbance and protein amount. Accurate measure of glucose in a serum. Upon arrival to the clinic, a blood sample is drawn. Glucose concentration in this sample is the individual"s fasting level of glucose in the blood: 2-hour sample: patient is given a standard glucose solution to drink.
